Reaction mass of 2,2'-(ethylenedioxy)diethanol and 3,6,9,12,15-pentaoxaheptadecane-1,17-diol and 3,6,9,12-tetraoxatetradecane-1,14-d

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

Prior to or in 1989

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: The study was conducted according to guideline and/or standard method but was non-GLP.

Justification for type of information:

Read across is based on the category approach. Please refer to attached category document.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1989

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: EPA Committee on Methods for Toxicity Tests with Aquatic Organisms

Deviations:

not specified

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Tetraethylene Glycol

IUPAC Name:

Tetraethylene Glycol

Constituent 2

Reference substance name:

3,6,9-trioxaundecane-1,11-diol

EC Number:

203-989-9

EC Name:

3,6,9-trioxaundecane-1,11-diol

Cas Number:

112-60-7

Molecular formula:

C8H18O5

IUPAC Name:

2,2'-[oxybis(ethane-2,1-diyloxy)]diethanol

Test material form:

not specified

Details on test material:

Tetraethylene glycol was used. No additional information available.

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
Not applicable

Sampling and analysis

Analytical monitoring:

no

Details on sampling:

Not applicable

Test solutions

Vehicle:

no

Details on test solutions:

Stock solutions preparation and stability: Dissolution in dilution water
Exposure vessel type: 250 ml beakers with 200 ml test solution
Dilution water source: Kanawha River water, Charleston, West Virginia
Dilution water chemistry: Hardness 40-60 mg/L as CaCO3. Alkalinity 25-38 mg/L as CaCO3. pH 7.0-7.2. Conductivity 250 umhos/cm
Water chemistry (DO, pH) in test in the control and at least one concentration where effects were observed. pH = 6.9 –7.2 throughout test. DO was greater than 8.0 throughout test

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

Source: Culture maintained in the laboratory for several years, initially stocked from the EPA laboratory at Duluth, Minnesota
Age at study initiation: Neonates <24 hours old were used

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Post exposure observation period:

No additional information available.

Test conditions

Hardness:

Hardness 40-60 mg/L as CaCO3

Test temperature:

20 ± 2C

pH:

7.0-7.2

Dissolved oxygen:

DO was greater than 8.0 throughout test

Salinity:

No data

Nominal and measured concentrations:

0 (control), 3600, 6000, and 10,000 mg tetraethylene glycol/L

Details on test conditions:

Test design: 4 replicates per control and treatment, 5 test organisms per replicate.
Method of calculating mean measured concentrations: Nominal concentrations were used.
Exposure period: 48 hours
Analytical monitoring: Nominal concentrations were used

Reference substance (positive control):

not specified

Results and discussion

Effect concentrations

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

7 746 mg/L

Details on results:

Remarks: Biological results: After 24 hours, there was 0% immobilisation in the controls and in the 3600 and 6000 mg/L treatments, and 85% immobilisation in the 10,000 mg/L treatment. After 48 hours, there was 0% immobilisation in the controls and in the 3600 and 6000 mg/L treatments, and 100% immobilisation in the 10,000 mg/L treatment

Results with reference substance (positive control):

Not applicable

Reported statistics and error estimates:

Statistical results: LC50 = 7746 mg/L

Any other information on results incl. tables

No additional information available.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Tetraethylene glycol is practically non-toxic to Daphnia magna. LC50 = 7746 mg/L

Executive summary:

The acute toxicity of tetraethylene glycol was examined with daphnia. After 24 hours, there was 0% immobilisation in the controls and in the 3600 and 6000 mg/L treatments, and 85% immobilisation in the 10,000 mg/L treatment. After 48 hours, there was 0% immobilisation in the controls and in the 3600 and 6000 mg/L treatments, and 100% immobilisation in the 10,000 mg/L treatment. The acute LC₅₀ in daphnia was 7746 mg/L in this test.