Reaction mass of 2,2'-(ethylenedioxy)diethanol and 3,6,9,12,15-pentaoxaheptadecane-1,17-diol and 3,6,9,12-tetraoxatetradecane-1,14-d

Long-term toxicity to fish

Administrative data

Endpoint:

fish early-life stage toxicity

Type of information:

read-across based on grouping of substances (category approach)

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Study conducted according to ASTM standards.

Justification for type of information:

Read across is based on the category approach. Please refer to attached category document.

Data source

Reference

Reference Type:

publication

Title:

Unnamed

Year:

1985

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: ASTM E-47.01

Principles of method if other than guideline:

- Method: ASTM E-47.01, Draft No. 3, 1980 (Standard for Toxicity Tests With the Early Life Stages of Fisheries)

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

2,2'-(ethylenedioxy)diethanol

EC Number:

203-953-2

EC Name:

2,2'-(ethylenedioxy)diethanol

Cas Number:

112-27-6

Molecular formula:

C6H14O4

IUPAC Name:

2,2'-[ethane-1,2-diylbis(oxy)]diethanol

Details on test material:

- Name of test substance (as cited in study report): Triethylene Glycol (TEG)

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Menidia peninsulae

Details on test organisms:

TEST ORGANISM
- Common name: tidewater silverside

Study design

Test type:

flow-through

Water media type:

saltwater

Limit test:

no

Total exposure duration:

28 d

Test conditions

Test temperature:

25 +/- 2 °C

pH:

7.69 - 7.73

Dissolved oxygen:

7.1 - 7.2 mg/L

Salinity:

20 +/- 2 parts per hundred

Nominal and measured concentrations:

- Concentrations (mg/L): 3.2, 10, 32, 100, 320, 1000 (nominal)
- Concentrations (mg/L): ND*, ND*, 15, 40, 230, 1500 (measured)

* Nondetectable (< 10.0 mg/L)

Details on test conditions:

TEST CONDITIONS
- Sea water from Santa Rosa Sound was filtered (20-µg filter), aerated, heated in a reservoir to required temperature and delivered to test aquaria
- Salinity was maintained by dilution of natural seawater with deionized freshwater

TEST SYSTEM
- Fish embryos were collected from filter floss on which spawning occured
- Embryos were randomly placed in incubation cups
- Two incubation cups were placed in each of two duplicate 2-L aquaria per treatment
- TEG was introduced with dosing apparatus which delivered 1 L of seawater to each treatment at 5 cycles/h

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- survival of embryos to hatching, survival of fry, and fish weight at 28 days were monitored

Results and discussion

Effect concentrations

Duration:

28 d

Dose descriptor:

LC50

Effect conc.:

> 1 500 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mortality

Details on results:

Statistically significant differences were found in weight and survival at the highest test concentrations (230 and 1500 mg/L).
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Weight:
Average weight of fish exposed to 230 mg/L was 18 % less than average control weight, while average weight of fish exposed to 1500 mg/L was 26% less than average control weight.

Survival:
In the 1500 mg/L treatment, the significant effect in combined survival (embryo and fry) was 36 % less than that of the control. Survival of embryos was significantly different from the control at 230 mg/L but not at 1500 mg/L. This suggests that the effect observed in the 230 mg/L treatment may not be caused by toxicity of the test item because the response was not concentration dependent.

Any other information on results incl. tables

Concentration [mg/L]		Survival [%]			Average	Percentage of
nominal	measured	Embryos	Fry	Combined	Wet Weight [mg]	Control Weight
control	ND	100	83	83	13.2	-
3,2	ND	94	93	83	14.2	109
10	ND	98	89	88	12.4	94
32	15	100	62	62	13.9	105
100	40	94	81	75	12.1	92
320	230	91	86	78	10.8	82
1000	1500	97	55	53	9.7	73