Reaction mass of 2,2'-(ethylenedioxy)diethanol and 3,6,9,12,15-pentaoxaheptadecane-1,17-diol and 3,6,9,12-tetraoxatetradecane-1,14-d

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

No data.

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: A Non-GLP study conducted by following EPA-ASTM procedure.

Justification for type of information:

Read across is based on the category approach. Please refer to attached category document.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1983

Report date:

1983

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

other: Methods for Acute Toxicity Tests with Fish, Macro invertebrates and Amphibians, Ecological Research Series, EPA-600/3-75-009, April, 1975

Deviations:

not specified

Test guideline 2

Qualifier:

according to guideline

Guideline:

other: Methods for Measuring the Acute Toxicity of Effluents to Aquatic Organisms Environmental Monitoring Series, EPA-600/4-78-012.

Deviations:

not specified

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

2,2'-(ethylenedioxy)diethanol

EC Number:

203-953-2

EC Name:

2,2'-(ethylenedioxy)diethanol

Cas Number:

112-27-6

Molecular formula:

C6H14O4

IUPAC Name:

2,2'-[ethane-1,2-diylbis(oxy)]diethanol

Test material form:

not specified

Details on test material:

No additional information available.

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
Not applicable.

Sampling and analysis

Analytical monitoring:

no

Details on sampling:

Not applicable.

Test solutions

Vehicle:

not specified

Details on test solutions:

Chareston (West Virginia) municipal water (dilution water in tests) was treated with activated carbon in a series of two 8-10 gal. filters which yields a chlorine-free dilution water with pH of 7.0 to 7.4. Total hardness of this water measured 40 to 60 mg/L as CaC03. Water quality was checked frequently and the carbon beds are changed at 8-10 month intervals. Temperature adjustment tank maintains water at about 18-20°C.

Test organisms

Test organisms (species):

Pimephales promelas

Details on test organisms:

The test fish (fathead minnows) are obtained from the Kurtz Fish Hatchery in eastern Pennsylvania and acclimated to their new environment for about 15 days prior to use in a test. Size of the minnows ranged from 3-6 cm. Any chemical treatment required to control or prevent disease was stopped at least five days prior to using the fish in tests.

Study design

Test type:

not specified

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Post exposure observation period:

No additional data available.

Test conditions

Hardness:

Total hardness of this water measured 40 to 60 mg/L as CaC03.

Test temperature:

Temperature adjustment tank maintains water at about 18-20°C.

pH:

pH of 7.0 to 7.4 prior to adding test material

Dissolved oxygen:

Dissolved oxygen levels were monitored during the test period of 96 hr. Minimal aeration was supplied when the dissolved oxygen was below
about 4 mg/L.

Salinity:

No data.

Nominal and measured concentrations:

At least 10, 000 mg/L (lower concentrations may have been tested but are not stated in report).

Details on test conditions:

The definitive tests were conducted by EPA-ASTM procedures (1,2) using 10 fathead minnows per test concentration in a total volume of 10 liters.

References:
1) Methods for Acute Toxicity Tests with Fish, Macro invertebrates and Amphibians, Ecological Research Series, EPA-600/3-75-009, April, 1975.

2) Methods for Measuring the Acute Toxicity of Effluents to Aquatic Organisms Environmental Monitoring Series, EPA-600/4-78-012.

Reference substance (positive control):

yes

Remarks:

ethylene oxide

Results and discussion

Effect concentrations

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 10 g/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

The 96 hour LC50 was >10,000 mg/L

Results with reference substance (positive control):

The 96 hour LC50 of ethylene oxide in fathead minnows was 84 mg/L.

Reported statistics and error estimates:

No additional information available.

Any other information on results incl. tables

Sublethal observations / clinical signs:

No additional information available.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

96 hour LC50 value concluded to be >10000 mg/L.

Executive summary:

The 96 hour LC50 was determined with the fathead minnow. The 96 hour LC50 value was calculated to be >10,000 mg/L.