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EC number: 279-256-2 | CAS number: 79771-28-1
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 96 hours
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Qualifier:
- according to guideline
- Guideline:
- other: ASTM E 729-780 (1980) (Standard Practice for conducting acute toxicity tests with fishes, macroinvertebrate, and amphibians)
- GLP compliance:
- yes
- Test organisms (species):
- Cyprinus carpio
- Details on test organisms:
- - Common name: Common carp
- Source: Wu Sha Tow Fresh Water Fish Culture Demonstration Center, R.O.C.
- Age at study initiation: 60-90 days old
- Length at study initiation: 2-4 cm
- Weight at study initiation: 2.0 ± 0.1 g
- Acclimation period: at least seven days - Test type:
- semi-static
- Water media type:
- freshwater
- Total exposure duration:
- 96 h
- Test temperature:
- 25 °C
- Details on test conditions:
- - Test vessel: Glass tank, 23 cm (id) × 30 cm (h)
- Test volume: 10 L
- Aeration: Gently
- Renewal rate of test solution: 24 hours
- Photoperiod: 12-hours light - Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Sublethal observations / clinical signs:
Table 1. Survival of common carp (Cyprinus carpio) exposed to EVERZOL BLUE ED-G for 96 hours in preliminary test
Nominal Concentration
(mg/L)
No. of Survival Fish
0 hr
3 hr
24 hr
48 hr
72 hr
96 hr
100
10
10
10
10
10
10
10
10
10
10
10
10
10
1.0
10
10
10
10
10
10
Control
10
10
10
10
10
10
Table 2. Survival of common carp (Cyprinus carpio) exposed to EVERZOL BLUE ED-G for 96 hoursin definitive test
Nominal Concentration
(mg/L)
No. of Survival Fish*
0 hr
3 hr
24 hr
48 hr
72 hr
96 hr
100
30
30
30
30
30
30
Control
30
30
30
30
30
30
*: There was no abnormal behavior observed.
Table 3. Water quality measurements during the acute toxicity test of EVERZOL BLUE ED-G to common carp (Cyprinus carpio) in definitive test
Nominal Concentration
(mg/L)
Exposure Time
3 hr
24 hr
48 hr
72 hr
96 hr
pH
100
8.4 ± 0.0
8.4 ± 0.0
8.3 ± 0.0
8.4 ± 0.1
8.4 ± 0.0
Control
8.4 ± 0.0
8.3 ± 0.0
8.3 ± 0.0
8.3 ± 0.0
8.3 ± 0.1
dissolved oxygen (mg/L)*
100
6.8 ± 0.0
7.0 ± 0.3
6.9 ± 0.3
7.7 ± 0.1
7.4 ± 0.2
Control
6.9 ± 0.2
6.6 ± 0.3
7.1 ± 0.8
7.5 ± 0.1
7.4 ± 0.1
conductivity (μscm-1)
100
544 ± 10
561 ± 5
523 ± 14
524 ± 5
516 ± 25
Control
507 ± 8
529 ± 5
483 ± 7
489 ± 10
481 ± 17
mg/L calcium as CaCO3
100
57 ± 3
58 ± 5
57 ± 1
50 ± 2
58 ± 2
Control
59 ± 1
59 ± 1
58 ± 1
54 ± 1
56 ± 0
*: Air saturation value at 25°C: 8.26 mg/L
Table 4. The acute toxicity of EVERZOL BLUE ED-G to common carp (Cyprinus carpio)
Parameter
Exposure Time
24 hr
48 hr
72 hr
96 hr
LC50
> 100
> 100
> 100
> 100
- Conclusions:
- According to ASTM E 729-780 and OECD 202 test method, the 96-hr LC50 of EVERZOL BLUE ED-G was >100 mg/L.
No adverse effects were reported. - Executive summary:
This test using the procedures outlined in the TACTRI Study Plan for EF3G1090.7 (07AQC321) which is based on the ASTM E 729-780 (ASTM, 1980) and OECD 202 (OECD, 1981). The values of water quality measurements during definitive test were considered adequate for testing. Throughout the test period, the colors of all the test solutions were deep blue except controls treatment. There was no abnormal behavior observed in all exposure levels during testing. Under the conditions of this test, the 96-hr LC50 of EVERZOL BLUE ED-G was >100 mg/L.
Reference
Description of key information
The 96-hr LC50 of EVERZOL BLUE ED-G was >100 mg/L for common carp (ASTM E 729-780 and OECD TG202).
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 100 mg/L
Additional information
The values of water quality measurements contain pH (8.3-8.4), dissolved oxygen concentrations (6.6-7.7 mg/L), conductivity (481-561 μscm-1) and total hardness (50-59 mg/L as CaCO3) during definitive test were considered adequate for testing. Throughout the test period, the colors of all the test solutions were deep blue except controls treatment. There was no abnormal behavior observed in all exposure levels during testing. Under the conditions of this test, the 96-hr LC50 of EVERZOL BLUE ED-G was >100 mg/L.
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