Pyridinium, 1,1'-[(6,13-dichloro-4,11-disulfo-3,10-triphenodioxazinediyl)bis[imino-2,1-ethanediylimino[6-[(2,5-disulfophenyl)amino]-1,3,5-triazine-4,2-diyl]]]bis[3-carboxy-, dihydroxide, bis(inner salt), hexasodium salt

Adsorption / desorption

Link to relevant study record(s)

Reference

Endpoint:

adsorption / desorption, other

Type of information:

experimental study

Adequacy of study:

key study

Study period:

From June 13, 2016 to December 01, 2016

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 121 (Estimation of the Adsorption Coefficient (Koc) on Soil and on Sewage Sludge using High Performance Liquid Chromatography (HPLC))

GLP compliance:

yes

Type of method:

HPLC estimation method

Media:

soil/sewage sludge

Details on study design: HPLC method:

- Apparatus: Agilent-1200 Series High Performance Liquid Chromatography
- Type, material and dimension of analytical (guard) column: BDS HYPERSIL Cyano Column, 5μm, 150 × 4.6 mm (Thermo)
- Detection system: Agilent-1260 Infinity Photodiode Array Detector

Type:

Koc

Remarks:

on sewage sludge

Value:

< 28.84

Type:

Koc

Remarks:

on soil

Value:

< 18.2

Table 1. The logarithm of adsorption coefficient (log K_OC) on sewage sludge and on soil and the logarithm of capacity factor (log K′) of control article and reference article

Article name	The logarithm of adsorption coefficient (log KOC)		Retention time (min)		Average retention time (min)	Capacity factor (K′)	The logarithm of capacity factor (log K′)
	Sewage sludge	Soil	t1	t2
Control article
Formamide	─	─	3.031	3.026	3.029	NA	NA
Reference article
Acetanilide	1.52	1.26	4.900	4.897	4.899	0.617	-0.209
Atrazine	1.66	1.81	7.964	7.967	7.966	1.630	0.212
Monuron	1.46	1.99	7.617	7.617	7.617	1.515	0.180
2,5-Dichloroaniline	2.45	2.55	8.521	8.522	8.522	1.814	0.259
Benzoic acid phenylester	3.26	2.87	11.378	11.383	11.381	2.758	0.441
Fenthion	3.75	3.31	13.819	13.827	13.823	3.564	0.552
Phenanthrene	4.35	4.09	13.397	13.407	13.402	3.425	0.535

NA: Not application

Table 2. Retention time and capacity factor (K′) of test article “Everzol SB26”

Peak	Retention time (min)		Average retention time (min)	Average Capacity factor (K′)	The logarithm of capacity factor (log K′)
	t1	t2
1	2.491	2.495	2.493	-0.177	NA
2	2.644	2.648	2.646	-0.126	NA
3	3.025	3.030	3.028	0.000	NA

NA: Not application

Table 3. The adsorption coefficient (K_OC) on sewage sludge and on soil of test article “Everzol SB26”

Peak	The logarithm of adsorption coefficient (log KOC)		The adsorption coefficient (KOC)		Area of peak (%)
	Sewage sludge^a	Soil^b	Sewage sludge	Soil
1	< 1.46	< 1.26	< 28.84	< 18.20	23.09
2	< 1.46	< 1.26	< 28.84	< 18.20	20.98
3	< 1.46	< 1.26	< 28.84	< 18.20	55.94

^a Calibration curve of reference article on sewage sludge: y = 0.139 × -0.029

^b Calibration curve of reference article on soil: y = 0.330 × -0.520

Validity criteria fulfilled:

yes

Conclusions:

According to OECD 121 test method, the adsorption coefficients (KOC) of Everzol SB26 on sewage sludge and on soil were < 28.84 and < 18.20, respectively.

Executive summary:

This test using the procedures outlined in the SuperLab Study Plan for M62-151100137001EN which is based on the SOP for the OECD 121 (SOPE-507) and OECD 121 (OECD, 2001). The results of this OECD 121 test for Everzol SB26 show that all validity criteria were met. The adsorption coefficients (K_OC) of Everzol SB26 on sewage sludge and on soil were < 28.84 and < 18.20, respectively.

Description of key information

Respectively, the adsorption coefficients (K_OC) of Everzol SB26 on sewage sludge and on soil were < 28.84 and < 18.20 (OECD TG 121).

Key value for chemical safety assessment

Koc at 20 °C:

28.84

Other adsorption coefficients

Type:

log Kp (solids-water in soil)

Value in L/kg:

1.26

at the temperature of:

20 °C

Additional information

All of the articles were ran in duplicate and the difference of log K_OC of test article between duplicate were < 0.25.In order to confirm retention times of test article had not drifted on HPLC analysis, formamide as the control article was analyze before and after the test artic. All test validity criteria were met. The adsorption coefficients (K_OC) of Everzol SB26 on sewage sludge and on soil were < 28.84 and < 18.20, respectively.

[LogKoc: 1.46]