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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Acute Toxicity: other routes

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Administrative data

Endpoint:
acute toxicity: other routes
Adequacy of study:
supporting study
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
documentation insufficient for assessment
Remarks:
Extensive investigations, but insufficient documentation

Data source

Reference
Reference Type:
publication
Title:
Über die Erzeugung von Sarkomen durch die besonders gereinigten Triphenylmnethanfarbstof!e Lichtgrün SF und Patentblau AE bei der wiederholten subkutanen Injektion an der Ratte
Author:
Gross, E.
Year:
1961
Bibliographic source:
Zeitschrift für Krebsforschung, Vol 64, pp 287-304

Materials and methods

Principles of method if other than guideline:
intraperitoneal injection with post-observation period
GLP compliance:
no
Limit test:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Dihydrogen (ethyl)[4-[4-[ethyl(3-sulphonatobenzyl)]amino]-2'-sulphonatobenzhydrylidene]cyclohexa-2,5-dien-1-ylidene](3-sulphonatobenzyl)ammonium, disodium salt
EC Number:
223-339-8
EC Name:
Dihydrogen (ethyl)[4-[4-[ethyl(3-sulphonatobenzyl)]amino]-2'-sulphonatobenzhydrylidene]cyclohexa-2,5-dien-1-ylidene](3-sulphonatobenzyl)ammonium, disodium salt
Cas Number:
3844-45-9
Molecular formula:
C37 H34 N2 Na2 O9 S3
IUPAC Name:
disodium 2-({4-[ethyl(3-sulfonatobenzyl)amino]phenyl}{4-[ethyl(3-sulfonatobenzyl)iminio]cyclohexa-2,5-dien-1-ylidene}methyl)benzenesulfonate
Test material form:
solid - liquid: aqueous solution
Details on test material:
Test substance 37%ige Formulation of Basacid Blau ("Patentblau")
Other identifier: Colour index no. 671
Other identifier: Diäthyl-di-m-sulfobenzyl-di-p-amino-ortho-fuchsoniumonium (Na-Salz)
Identified by structural formula
The substance was provided in a form marked "particularly pure"
by-products: mainly sodium chloride, to a minor extent sodium sulfate

The pH of a 2% solution in water at 20°C was 5.43.

The formulation remains stable for several days as indicated by the constancy of the UV absorption spectrum and the colour.

Test animals

Species:
mouse
Strain:
not specified
Sex:
not specified
Details on test animals or test system and environmental conditions:
No data

Administration / exposure

Route of administration:
subcutaneous
Vehicle:
physiological saline
Doses:
4600 mg/kg bw
No. of animals per sex per dose:
no data

Results and discussion

Effect levels
Dose descriptor:
LD50
Effect level:
> 4 600 mg/kg bw
Other findings:
Parenteral application of the substance resulted quickly in a whole-body blue colouration with the exception of the lungs. The blue colouration persisted for a few days. Clearance of the colour was observed via the bile and the urine.

Applicant's summary and conclusion

Conclusions:
The substance is of low acute toxicity after intraperitoneal injection in mice.