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EC number: 921-728-3 | CAS number: 64741-66-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Adopted according to OECD SIDS (public available peer reviewed source) and respective study summary. The original source is not available and has not been reviewed.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Vehicle:
- no
- Details on test solutions:
- Individual treatment solutions were prepared by adding the test substance, via syringe, to 17 L of laboratory blend water in 20 L glass carboys that were sealed to minimize volatile loss of test material. The stock solutions were mixed for 24 hours with a vortex of <10% (1-2 cm vortex). Mixing was performed using a magnetic stir plate and teflon stir bar. After mixing, the solutions were allowed to settle for one hour and the Water Accommodated Fraction (WAF) was then siphoned from the bottom of the mixing vessel and added to the test vessels. Siphons were placed in the carboys prior to adding the test material.
- Test organisms (species):
- Oncorhynchus mykiss (previous name: Salmo gairdneri)
- Details on test organisms:
- TEST ORGANISM
- Common name: rainbow trout
- Source: Thomas Fish Co.
- Age at study initiation (mean and range, SD): approximately 5 weeks
- Length at study initiation (length definition, mean, range and SD): mean total length = 3.4 cm
- Weight at study initiation (mean and range, SD): mean wt. = 0.297 g - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Test temperature:
- 15.4 – 16.0°C
- pH:
- 7.5 - 8.7 (7.6 to 8.7 for "new" solutions and 7.5 to 8.4 for "old" solutions)
- Dissolved oxygen:
- 5.0 – 9.7 mg/L (8.0 to 9.7 mg/L for "new" solutions and 5.4 to 7.7 mg/L for "old" solutions)
- Nominal and measured concentrations:
- 0, 0.9, 2.0, 10.0, 22.0, and 50.0 mg/L (nominal)
non-detected, 0.05, 0.12, 0.33, 0.36, 0.47 mg/L (measured) - Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Type: closed
- Material, size, headspace, fill volume: 4.0 L aspirator bottles containing 4.5L of solution sealed with no headspace
- Renewal rate of test solution (frequency/flow rate): Approximately 80% of each solution was renewed daily from a freshly prepared WAF.
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 3
- Biomass loading rate: 0.46 g fish/L
OTHER TEST CONDITIONS
- Photoperiod: 16 hrs light and 8 hrs dark
- Light intensity: 73 ft. candles - Reference substance (positive control):
- no
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LL50
- Effect conc.:
- 18.4 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- other: Water Accomodated Fraction (WAF)
- Basis for effect:
- mortality (fish)
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 0.11 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- other: Water Accomodated Fraction (WAF)
- Basis for effect:
- mortality (fish)
- Reported statistics and error estimates:
- Probit procedure of SAS
- Sublethal observations / clinical signs:
Tab.: Fish Total Mortality
Nominal Loading
(mg/L)
Fish Total Mortality
24h
48h
72h
96h
Control
0
1
1
1
0.9
0
2
2
0
2.0
0
2
2
1*
10.0
0
7
7
8
22.0
1
9
9
11
50.0
6
13*
21*
21*
* 2 non-test related mortalities
- Validity criteria fulfilled:
- not specified
- Conclusions:
- The acute toxicity, as measured by mortality to rainbow trout (Oncorhynchus mykiss), was evaluated in freshwater. Under the conditions of this study, the test substance produced acute lethal toxicity to Oncorhynchus mykiss at 18.4 mg/L, based on nominal loading of the test substance in water.
- Executive summary:
The acute toxicity, as measured by mortality to rainbow trout (Oncorhynchus mykiss), was evaluated in freshwater. Under the conditions of this study, the test substance produced acute lethal toxicity to Oncorhynchus mykiss at 18.4 mg/L, based on nominal loading of the test substance in water.
Reference
Description of key information
LL50 (96 h) = 18.4 mg/L (Oncorhynchus mykiss)
Key value for chemical safety assessment
Additional information
A study conducted according to OECD 203 guideline is available on the acute toxicity to fish for hydrocarbons, C7-C9, isoalkanes (CEFIC 1995). The acute toxicity, as measured by mortality to rainbow trout (Oncorhynchus mykiss), was evaluated in freshwater by using water accomodated fractions (WAFs).
In this methodology, different loadings of the substance are added to the test medium and equilibrium between the water and the hydrocarbons is achieved according to their specific water solubility. The hydrocarbon concentrations at equilibrium will be characteristic of the loading of the substance, and therefore toxicity values from WAF studies are expressed as effect loadings or lethal loadings (EL or LL), not as concentrations. These effect loadings are used to determine environmental classifications. The WAF methodology is widely accepted for the testing of complex hydrocarbon substances and other UVCBs, and it has been incorporated in different guidance documents, including the REACH guidance (see draft decision response letter in Section 13).
Under the conditions of this study, the test substance, hydrocarbons, C7-C9, isoalkanes produced acute lethal toxicity to Oncorhynchus mykiss at 18.4 mg/L (CEFIC 1995) based on nominal loadings of the test substance in water.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.

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