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EC number: 221-998-6 | CAS number: 3312-04-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Skin irritation / corrosion
Administrative data
- Endpoint:
- skin corrosion: in vitro / ex vivo
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- secondary literature
- Justification for type of information:
- Data is from safety assessment reports
Data source
Reference
- Reference Type:
- secondary source
- Title:
- OPINION ON UV filter S86 - Phenylene bis-diphenyltriazine
- Author:
- Scientific Committee on Consumer Safety - SCCS
- Year:
- 2 015
- Bibliographic source:
- Scientific Committee on Consumer Safety - SCCS, OPINION ON UV filter S86 - Phenylene bis-diphenyltriazine ; 2015
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 431 (In Vitro Skin Corrosion: Reconstructed Human Epidermis (RHE) Test Method)
- Version / remarks:
- Annex IB.40, Directive 2000/33/CE from the Commission, April 25th 2000.
- Principles of method if other than guideline:
- To assess the dermal irritation potential of 3,3'-(1,4-Phenylene)bis(5,6-diphenyl-1,2,4-triazine) using Reconstituted Human Epidermis (RHE) made from Human Normal Keratinocytes (SkinethicTM).
- GLP compliance:
- not specified
Test material
- Reference substance name:
- 3,3'-(1,4-Phenylene)bis(5,6-diphenyl-1,2,4-triazine)
- Cas Number:
- 55514-22-2
- Molecular formula:
- C36H24N6
- IUPAC Name:
- 3,3'-(1,4-Phenylene)bis(5,6-diphenyl-1,2,4-triazine)
- Test material form:
- solid
- Details on test material:
- Name of the test chemical: 3,3'-(1,4-Phenylene)bis(5,6-diphenyl-1,2,4-triazine)
Molecular Formula: C36H24N6
Molecular Weight: 540 g/mol
Substance Type: Organic
Physical State: Solid
Constituent 1
- Specific details on test material used for the study:
- Name of the test chemical: 3,3'-(1,4-Phenylene)bis(5,6-diphenyl-1,2,4-triazine)
Molecular Formula: C36H24N6
Molecular Weight: 540 g/mol
Substance Type: Organic
Physical State: Solid
Test substance: WP30
Test batch: LP110
Purity: 99.6% (HPLC, 320 nm)
In vitro test system
- Test system:
- human skin model
- Source species:
- human
- Cell type:
- non-transformed keratinocytes
- Cell source:
- foreskin from a single donor
- Source strain:
- other: human
- Details on animal used as source of test system:
- - Source: 0.5 cm2 Reconstituted Human Epidermis (RHE) made from Human Normal Keratinocytes (SkinethicTM). Abdominal skin from a 48-year-old woman.
- Type: Reconstituted Human Epidermis (RHE) made from Human Normal Keratinocytes (SkinethicTM). - Justification for test system used:
- no data available
- Vehicle:
- other: Paraffin oil
- Details on test system:
- concentration - 10%
No of replicates: 1
No of applications: 3 repeated applications
Treatment period: 20hours - Control samples:
- yes, concurrent negative control
- yes, concurrent positive control
- Amount/concentration applied:
- 10% in paraffin oil
- Duration of treatment / exposure:
- 3 repeated applications
- Duration of post-treatment incubation (if applicable):
- no data
- Number of replicates:
- 1
Test animals
- Species:
- other: not specified
- Strain:
- not specified
- Details on test animals or test system and environmental conditions:
- no data available
Test system
- Amount / concentration applied:
- no data available
- Duration of treatment / exposure:
- no data available
- Observation period:
- no data available
- Number of animals:
- no data available
- Details on study design:
- no data available
Results and discussion
In vitro
Results
- Irritation / corrosion parameter:
- % tissue viability
- Run / experiment:
- single run
- Value:
- 100
- Vehicle controls validity:
- not specified
- Negative controls validity:
- valid
- Positive controls validity:
- valid
- Remarks on result:
- no indication of irritation
- Other effects / acceptance of results:
- 3,3'-(1,4-Phenylene)bis(5,6-diphenyl-1,2,4-triazine) does not reduce MTT in a specific way. At the concentration of 10%, the cell viability was 100%.
Applicant's summary and conclusion
- Interpretation of results:
- other: not irritating
- Conclusions:
- 3,3'-(1,4-Phenylene)bis(5,6-diphenyl-1,2,4-triazine) does not reduce MTT in a specific way. At the concentration of 10%, the cell viability was 100%. Hence, 3,3'-(1,4-Phenylene)bis(5,6-diphenyl-1,2,4-triazine) was considered not a skin irritant.
- Executive summary:
The dermal irritation potential of 3,3'-(1,4-Phenylene)bis(5,6-diphenyl-1,2,4-triazine) was assessed using Reconstituted Human Epidermis (RHE) made from Human Normal Keratinocytes (SkinethicTM). The test was performed according to the Guidelines mentioned inAnnex IB.40, Directive 2000/33/CE from the Commission, April 25th 2000.
3 repeated applications of10% test chemical in paraffin was done to the0.5 cm2 Reconstituted Human Epidermis (RHE) made from Human Normal Keratinocytes (SkinethicTM). Abdominal skin from a 48-year-old woman was used. The treatment was continued for 20 hours.5% SDS in water and Crème reparatrice cicalfate, Avéne Laboratory, batch F2268, pure were used as positive and negative controls respectively. The MTT method was used forin vitrocytotoxicity testing. An MTT direct-interaction test was made before the main test on reconstructed epidermis.
The test chemical did not interact with MTT.
3,3'-(1,4-Phenylene)bis(5,6-diphenyl-1,2,4-triazine) does not reduce MTT in a specific way. At the concentration of 10%, the cell viability was 100%. Hence,3,3'-(1,4-Phenylene)bis(5,6-diphenyl-1,2,4-triazine) was considerednot a skin irritant.
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