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Toxicological information

Skin irritation / corrosion

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Administrative data

Endpoint:
skin irritation: in vivo
Type of information:
migrated information: read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Acceptable for assessment

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1973

Materials and methods

Principles of method if other than guideline:
BASF test:
1 ml of the test substance was applied to the skin of two rabbits. Readings were performed using a BASF scheme which is convertable into the Draize system (s. Details on study design)
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Cyclohexene
EC Number:
203-807-8
EC Name:
Cyclohexene
Cas Number:
110-83-8
Molecular formula:
C6H10
IUPAC Name:
cyclohexene

Test animals

Species:
rabbit

Test system

Type of coverage:
occlusive
Amount / concentration applied:
TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 1 ml
Duration of treatment / exposure:
20 hours
Observation period:
8 days
Number of animals:
2
Details on study design:
TEST SITE
- Area of exposure: 2.5 X 2.5 cm

REMOVAL OF TEST SUBSTANCE
- Washing (if done): no washing done


SCORING SYSTEM:
BASF scheme
Erythema:
(+) corresponds to Draize score 1
R+ corresponds to Draize score 2
R++ corresponds to Draize score 3
R+++ corresponds to Draize score 4
Edema
Ö(+) corresponds to Draize score 1
Ö+ vorresponds to Draize score 2
Ö++ corresponds to Draize score 3
Sack Ö+++ corresponds to Draize score 4
Sch+ slight scales
Sch++ rhagade-like scales

Readings were performed 24 hours, 48 hours 5, 7 and 8 days after the application of the test substance.

Results and discussion

In vivo

Resultsopen allclose all
Irritation parameter:
erythema score
Basis:
mean
Time point:
other: 24 and 48 hours
Score:
1.75
Max. score:
4
Reversibility:
not fully reversible within: 8 days (only animal 1)
Irritation parameter:
edema score
Basis:
mean
Time point:
other: 24 and 48 hours (5 days)
Score:
2
Max. score:
4
Reversibility:
not fully reversible within: 8 days
Remarks on result:
other: for animal 1 the 5 day score was considered instead of the 48 hours score; slight scaling was observed after 7 and 8 days

Any other information on results incl. tables

 

Animal 1

Animal 2

Mean

 

Erythema

Ed.

Erythema

Edema

Ery.

Ed.

24 hours

1

2

2

2

1.5

2

48 hours

2

unknown

2

2

2

-

5 days

2

2

2

2

2

2

7 days

2

slight scaling

0

slight scaling

1

-

8 days

1

 

slight scaling

0

slight scaling

0.5

-

Mean 24 and 48 hours

1.5

-

2

2

 

 

Applicant's summary and conclusion