1,2,3-trichlorobenzene

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Study period:

From: 12.1.1982 To: 2.2.1982

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Comparable to guideline study with accepatable restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1982

Report Date:

1982

Materials and methods

Test guideline

Qualifier:

equivalent or similar to

Guideline:

OECD Guideline 401 (Acute Oral Toxicity)

Deviations:

yes

Remarks:

No analytical purity reported. No GLP. Only 2 dosis tested.

GLP compliance:

not specified

Test type:

standard acute method

Limit test:

yes

Test material

Reference

Name:

Unnamed

Type:

Constituent

Details on test material:

- Name of test material (as cited in study report): 1,2,3-trichlorobenzene solidified melted
- Physical state: solid
- Analytical purity: not reported

Test animals

Species:

rat

Strain:

Wistar

Sex:

male/female

Details on test animals and environmental conditions:

TEST ANIMALS
- Source: Sparte, OC, Germany
- Age at study initiation: 9-14 weeks old
- Weight at study initiation: 182 (males), 177 (females)
- Fasting period before study: from 16 hours before the application to 4 hours after the application
- Housing: Makrolon cages type III
- Diet (e.g. ad libitum): ad libitum (except during the fasting period)
- Water (e.g. ad libitum): ad libitum

ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22 ± 1.5 ° C
- Humidity (%): 60 ± 5%
- Photoperiod (hrs dark / hrs light): 12 hrs dark / 12 hrs light (7 a.m-7 p.m)


IN-LIFE DATES: From: 12.1.1982 To: 2.2.1982

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

other: Lutrol

Details on oral exposure:

MAXIMUM DOSE VOLUME APPLIED: 20 mL/kg bw

Doses:

3100 and 5000 mg/kg bw

No. of animals per sex per dose:

5

Control animals:

other: not applicable

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: At the day of the treatment the animals were controlled several times. During the 14-day observation period they were monitored twice per day ( once per day over weekends and holidays). The body weights were recorded at the day of the treatment, after 1 week and the end of the observation period.
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight,organ weights, gross pathology

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

> 5 000 mg/kg bw

Remarks on result:

other: 95% CL: Not applicable

Mortality:

No mortality was observed in the male and female group dosed with 3100  mg/kg bw 2/5 males and 2/5 females dosed with 5000 mg/kg bw died within the first  week post application.

Clinical signs:

Rats of both sex and in both dose groups suffered from weight loss, rough  fur, bloody snout and reduced general condition.

Body weight:

weight loss was reported

Gross pathology:

Gross autopsy of some of the surviving rats showed no pathological  findings.

Any other information on results incl. tables

Dosis	Toxicologicalresults			Time ofdeath	Start ofthesymptoms
mL/kg	Deadanimals	Animalswithsymptoms	Total ofanimalemployed
Males
3.1	0	3	5		7 d
5.0	2	5	5	2-3d	24 h
Females
3.1	0	4	5
5.0	2	5	5	2-6 d	24 h

 

 

Table 1.Effect of acute oral toxicity test

Applicant's summary and conclusion

Executive summary:

Löser E (1982)

In order to test the acute oral toxicity of 1,2,3 -trichlorobenzene, 5 male and 5 female tested were dosed by gavage (3100 and 5000 mg/kg bw) with a method similar to OECD guideline 401 with deviations (No analytical purity reported. No GLP. Only 2 dosis tested)

Tthe following toxicological effects were observed:

1) Mortality: no mortality was observed in the male and female group dosed with 3100  mg/kg bw 2/5 males and 2/5 females dosed with 5000 mg/kg bw died within the first  week post application.

2) Signs of intoxication
Rats of both sex and in both dose groups suffered from weight loss, rough  fur, bloody snout and reduced general condition.

3) Gross autopsy 
Gross autopsy of some of the surviving rats showed no pathological  findings.