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EC number: 925-653-7 | CAS number: -
Number of Immobilized Daphnia magna
Loading Rate (mg/l)
The purpose of this experiment was to determine the acute toxicity of low aromatic white spirits to Daphnia magna. 10 daphnia per test vessel (2 test vessels per concentration) were exposed to loading rates of 0, 1, 2.2, 4.6, 10, 22, 46, or 100 mg/l (WAF) of test substance. The exposure lasted for 48 hrs, and there was no renewal of test solution. At 24 and 48 hrs, the Daphnia were observed for immobilization. Immobilization was seen at test concentrations of 10 mg/l (WAF) and above, with immobilization of all daphnids at the 46 and 100 mg/l concentrations by the 24 hr observation. The 48-hr EL50 range for an invertebrate, Daphnia magna, was 10-22 mg/L (WAF).
The acute toxicity of water accomodated fractions (aqueous extrats) of Shellsol H has been determined to the crustacean zzoplakter Daphnia magna.
The test media were prepared by adding quantities of Shellsol H to culture medium appropriate to the test organisms and stirring in sealed vessels for 45 hours. The strirring period was demonstrated as being sufficient to ensure equilibration of the shellsol H and aqueous phases. After stirring the contents of the test vessels were left to settle for about 1 hour before drawing off the WAF for use as the test media in the toxicity tests.
The concentrations of dissolved hydrocarbons (derived from the shellsol H) in the test media was determined during the toxicit tests usig gas chromatography. The analyses showed that the concentration in the test media was <1%% of the loading rate. The reduction in the concentration of dissolved hydrocarbons in the test media during the test was ≤26%.
The acute toxicity to D. magna of water accomodated fractions of Shellsol H prepared at loading rates ranging from 10 to 1000 mg/l was determined in a 48 static toxicity test without renewal of the test media. The test was conducted in sealed test vessels. The 24 and 48 h EL50 values (loading rates resulting in mortality of 50% of the D. magna after 24 and 48 h exposure) were 460-1000 and 100 -220 mg/l respectively.
Water accommodated fractions of hydrocarbons, C9-C12, n-alkanes, isoalkanes, cyclics, 2-25% aromatics, produced a 48-hour EL50 value in the range 10 - 22 mg/L with Daphnia magna.
Water accommodated fractions of hydrocarbons, C10-C13, n-alkanes, isoalkanes, cyclics, 2-25% aromatics, produced a 48-hour EL50 value in the range 100 - 220 mg/L with Daphnia magna.
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