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Additional toxicological data

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Endpoint:
additional toxicological information
Type of information:
other: published data
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment
Justification for type of information:
Carbon disulphide (CAS number 75–15–0) is both reagents used in the manufacture of S-allyl O-pentyl dithiocarbonate. Therefore, Carbon disulphide (CAS number 75–15–0) need to be considered in the assessment of S-allyl O-pentyl dithiocarbonate.

Data source

Reference
Reference Type:
publication
Title:
Oxidation and phosphorylation processes in brain mitochondria of rats exposed to carbon disulfide.
Author:
Tarkowski S, and Sobczak H
Year:
1971
Bibliographic source:
J Neurochem 18: 177-182

Materials and methods

Type of study / information:
The effects of acute and long-term exposure to CS2, on oxidation and phosphorylation processes in brain mitochondria of rats were studied.
Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
The effects of acute and long-term exposure to CS2, on oxidation and phosphorylation processes in brain mitochondria of rats were studied.
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
Carbon disulphide
EC Number:
200-843-6
EC Name:
Carbon disulphide
Cas Number:
75-15-0
Molecular formula:
CS2
IUPAC Name:
dithioxomethane
Test material form:
aerosol dispenser: not specified
Details on test material:
Carbon disulphide (CAS number 75–15–0) is both reagents used in the manufacture of S-allyl O-pentyl dithiocarbonate. Therefore, Carbon disulphide (CAS number 75–15–0) need to be considered in the assessment of S-allyl O-pentyl dithiocarbonate..
- Name of test material (as cited in study report): Carbon disulfide

Results and discussion

Any other information on results incl. tables

CS2 intoxication exerted different signs of toxicity depending on the duration of treatment. After the 10-month exposure the animals experienced loss of motor equilibrium, muscular weakness, and hind-limb paresis. The acute challenging caused severe narcosis, reduced cardiac and respiratory rate, straightening of hind limbs, and lower body temperature. Nonetheless, the effects on the brain mitochondria were of the same type at both cases: disturbances in oxidative phosphorylation-uncoupling of oxidative phosphorylation, decreased phosphorus-oxygen (P:O) ratio, and a lower ATP-inorganic phosphorus (ATP-Pi) exchange rate.

Applicant's summary and conclusion

Conclusions:
CS2 poisoning (acute and chronic) to 803 and 578 ppm, respectively, resulted in similar disturbances in the brain mitochondria. The symtomps of poisoning were different for the two exposures.
Carbon disulphide (CAS number 75–15–0) is both reagents used in the manufacture of S-allyl O-pentyl dithiocarbonate. Therefore, Carbon disulphide (CAS number 75–15–0) need to be considered in the assessment of S-allyl O-pentyl dithiocarbonate.
Executive summary:

The effects of acute and long-term exposure to CS2, on oxidation and phosphorylation processes in brain mitochondria of rats were studied. Although rats developed different symptoms of poisoning, depending on the type of exposure, the brain mitochondria of both groups of animals exhibited the same types of disturbances in oxidative phosphorylation. The main characteristic of these disturbances was the uncoupling of oxidative phosphorylation indicated by lower respiratory control indices due to stimulation of oxidation of respiratory substrates by mitochondria in the metabolic state 4. This effect was accompanied by a decreased P:O ratio and a lower ATP-Pi exchange rate. An inhibitory effect of CS2 on the energy transfer processes is also suggested. The observed changes in oxidative phosphorylation were more distinct in the case of acute poisoning, with a longer period of an uninterrupted exposure enabling a more complete tissue saturation with CS2, than in the case of long-term exposure with shorter periods of intoxication within the day.