2-ethylhex-2-enal

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Guideline study, well documented.

Data source

Reference

Reference 1

Reference Type:

study report

Title:

Unnamed

Year:

1986

Report date:

1986

Reference 2

Reference Type:

other: reprint

Title:

Unnamed

Year:

2006

Report date:

1986

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

other: EEC Directive 79/831 Annex V Part C

Test guideline 2

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

2-ethylhex-2-enal

EC Number:

211-448-3

EC Name:

2-ethylhex-2-enal

Cas Number:

645-62-5

Molecular formula:

C8H14O

IUPAC Name:

2-ethylhex-2-enal

Details on test material:

- Name of test material (as cited in study report): 2-Ethylhexenal

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic (adaptation not specified)

Details on inoculum:

- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): activated sludge from municipal laboratory wastewater treatment plant
- Initial cell/biomass concentration: 30 mg/l

Duration of test (contact time):

31 d

Initial test substance concentration

Initial test substance concentration 1

Initial conc.:

43 mg/L

Based on:

test mat.

Initial test substance concentration 2

Initial conc.:

86 mg/L

Based on:

test mat.

Initial test substance concentration 3

Initial conc.:

171 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

TEST CONDITIONS
- Test temperature: 22 °C +- 1 °C

Reference substance

Reference substance:

aniline

Results and discussion

% Degradation

Parameter:

% degradation (O2 consumption)

Value:

ca. 50 - ca. 60

Sampling time:

31 d

Details on results:

- lag phase: 2 days
- log phase: 4 days

Biodegradation rates (%) of 2-Ethylhexenal during the 31 days test duration were as follows:

day RS IH PC TS1 TS2 TS3 TS4 TS5 TS6 TS7 mean
1 -7 0 0 -5 -4 -4 -13 -11 -3 -3 -6
4 6 35 1 29 33 33 26 20 37 37 31
7 66 - 2 42 45 46 38 28 46 46 42
14 70 - 3 52 57 57 51 39 54 54 52
21 71 - 3 52 57 58 51 39 54 54 52
28 74 - 5 54 59 60 55 44 56 56 55
31 74 - 5 55 60 62 56 44 57 57 56

Degradation rates refer to % BOD/ThOD (ThOD: 2794 mg/g)

RS: Reference substance
IH: Inhibition control
PC: Physico-chemical elimination control
TS 1,2,3: 86 mg/l test substance
TS 4,5 : 43 mg/l test Substance
TS 6,7 : 171 mg/l test substance

pH-values at the end of the test
pH
BC1 7.0
BC2 7.0
RS 8.1
IH 7.3
TS1 7.4
TS2 7.3
TS3 7.3
TS4 7.3
TS5 7.4
TS6 7.6
TS7 7.6
TS(median) 7.4

Kinetic of test substance (in %):
= 26 after 3 day(s)
= 41 after 7 day(s)
= 52 after 14 day(s)
= 52 after 21 day(s)
= 55 after 28 day(s)

BOD5 / COD results

Results with reference substance:

Kinetic of control substance (in %):
= 6 after 4 day(s)
= 66 after 6 day(s)

Applicant's summary and conclusion

Interpretation of results:

other: The test substance is not readily biodegradable under the test conditions.