2-ethylhex-2-enal

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Guideline study with acceptable restriction (e.g. non-GLP, no analytical verification of test item concentrations, solubiliser used)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1988

Report date:

1988

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: Directive 79/831/EEC, Annex V, Part C2

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

2-ethylhex-2-enal

EC Number:

211-448-3

EC Name:

2-ethylhex-2-enal

Cas Number:

645-62-5

Molecular formula:

C8H14O

IUPAC Name:

2-ethylhex-2-enal

Details on test material:

- Name of test material (as cited in study report): 2-Ethylhexen-2-al crude
- Analytical purity: >= 95 %)

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

yes

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: the test substance (100 mg/l) was dispersed with Tween 80 (100 mg/l; 95 %; Merck) in the test medium
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): Tween 80

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Source: originally supplied from "Institut National de Recherche Chimique appliquee, France", bred since 1978 at BASF
- Age at study initiation (mean and range, SD): 2-24 h

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Test temperature:

18.9-20.9 °C

pH:

7.52 - 7.99

Dissolved oxygen:

7.27 - 8.85

Nominal and measured concentrations:

Nominal test concentrations: 0 (control + solvent control), 1.563, 3.13, 6.25, 12.5, 25, 50, 100 mg/l

Details on test conditions:

TEST SYSTEM
- Test vessel: glass tubes with flat bottom
- Fill volume: 10 ml (2 ml/animal)
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4



TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: artificial freshwater: chlorine-free filtered tapwater supplemented with H2SO4 for the reduction of the buffering capacity of the carbonic acid system, and with deionized water for the reduction of the total hardness (total hardness: 2.70 +- 0.5 mmol/l, acid capacity to pH 4.3: 0.80 +- 0.10 mmol/l; molar ratio Ca:Mg = 4:1; molar ratio of Na:K = 10:1; conductivity: 550-650 µSiemens/cm; pH 7.7-8.3); aerated till saturation with oil-free air and allowed to stabilize for 24 h
- Intervals of water quality measurement: pH and dissolved oxygen: after 0 and 48 h


OTHER TEST CONDITIONS
- Light intensity: diffuse light


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Mobility after 0, 3, 6, 24 and 48 h

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

12.5 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

20.04 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 3

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

50 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Reported statistics and error estimates:

The EC50 values were calculated using the moving average method

Any other information on results incl. tables

Effect values (mg/l; 95 % confidence interval):
           24 h                 48 h
EC 0:      12.5                 12.5 
EC 50:     22.75 (9.24-36.26)   20.04 (12.2-27.88)
EC 100:    50                   50


Immobility:

Nominal                   Cumulative immobility (n)
Concentration (mg/l)   0 h   3 h    6 h   24 h   48 h
  0 (control)          0     1      1      1      1 
  0 (solvent control)  0     0      0      0      0 
  1.563                0     0      0      0      0 
  3.13                 0     0      0      0      0 
  6.25                 0     0      0      0      0 
 12.5                  0     0      0      0      1 
 25                    0     0      5     14      6 
 50                    0    15     15     20     20
100                    0    20     20     20     20

The results were obtained at 19.0 °C (test start), while the
test medium had the following specifications:
   pH 8.0
   Total hardness: 2.88 mmol/l
   Acid capacity to pH 4.3: 0.85 mmol/l

Nominal                    pH            oxygen (mg/l)
Concentration (mg/l)   0 h     48 h       0 h    48 h
  0 (control)          7.99    7.82       8.85   8.65
  0 (solvent control)  7.82    7.61       8.89   7.63
  1.563                7.95    7.83       8.70   8.73
  3.13                 7.93    7.78       8.63   8.52
  6.25                 7.93    7.76       8.70   8.39
 12.5                  7.90    7.67       8.85   7.67
 25                    7.86    7.67       8.73   8.22
 50                    7.79    7.6        8.75   8.02
100                    7.72    7.52       8.78   7.27