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EC number: 440-240-7 | CAS number: 1282554-35-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to soil macroorganisms except arthropods
Administrative data
- Endpoint:
- toxicity to soil macroorganisms except arthropods: short-term
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2009-08-04 to 2009-08-18
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study according to guideline OECD 207 .
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 010
- Report date:
- 2010
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 207 (Earthworm, Acute Toxicity Tests)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- 2-Propyl-4-methyl-6-(1-methylbenzimidazole-2-yl)benzimidazole
- IUPAC Name:
- 2-Propyl-4-methyl-6-(1-methylbenzimidazole-2-yl)benzimidazole
- Test material form:
- solid: particulate/powder
- Remarks:
- migrated information: powder
- Details on test material:
- - Storage condition of test material: storage room of the institute, preservation condition normal temperature
Constituent 1
Sampling and analysis
- Analytical monitoring:
- not specified
Test substrate
- Vehicle:
- yes
- Details on preparation and application of test substrate:
- - Method of mixing into soil (if used): test substance was dissolved in 2 ml methanol (concentration not specified) and mixed with fine quarz sand. After evaporation the mixture was mixed with the basic test substate.- Controls: yes- Chemical name of vehicle (organic solvent, emulsifier or dispersant): methanol- Concentration of vehicle in test medium (stock solution and final test solution): not specified- Evaporation of vehicle before use: yes
Test organisms
- Test organisms (species):
- Eisenia fetida
- Animal group:
- annelids
- Details on test organisms:
- TEST ORGANISM- Source: Shanghai Yinquan Agriculture develpment Co., Ltd (ID: EF-MKT-090511)- Age at test initiation (mean and range, SD): five month old adult earthworms from the same population- Weight at test initiation (mean and range, SD): 300 to 600 mgACCLIMATION- Acclimation period: at least 14 days quarantied prior to use in test, 7 days bred in cow dung soil prior to test- Acclimation conditions (same as test or not): 20+-2°C
Study design
- Study type:
- laboratory study
- Substrate type:
- artificial soil
- Limit test:
- yes
- Total exposure duration:
- 14 d
Test conditions
- Test temperature:
- 20-21°C
- pH:
- 7.12, determined at the test start
- Moisture:
- 32-35%
- Details on test conditions:
- TEST SYSTEM- Test container (material, size): 1L glas beaker - Amount of soil or substrate: 750 g test substrat- No. of organisms per container (treatment): 10- No. of replicates per treatment group: 4- No. of replicates per control: 4SOURCE AND PROPERTIES OF SUBSTRATE (if soil)Artificial soil test substrate was mixid by using 10 per cent sphagnum peat (pH 5.72, no visible plant remains, finely ground, moisture content of 3%)20 per cent kaolinite clay 70 per cent silica sand (particles of 0.2 mm diameter or lessMoisture content was adjusted with deionizid water or test solution to be 35 %OTHER TEST CONDITIONS- Photoperiod: 24 hours light- Temperature : 20°C to 21°CEFFECT PARAMETERS MEASURED (with observation intervals if applicable) :Lethal effects at 7 and 14 days,: weight and numbers of earthworms were determined at the begining and the end of the testAny adverse effect was recordedTemperature and moisture were measured at the beginning and the end of the testpH was measured at the beginning of the testTEST CONCENTRATIONS- Justification for using less concentrations than requested by guideline: based on the results of the range finding tests , the 14 d LC 50 was expected to be higher than 1000 mg/kg- Range finding study: yes, - Test concentrations: 10, 100, 1000 mg/kg dry artificial soil weight, highest concentration causes no mortality.- Results used to determine the conditions for the definitive study: yes
- Nominal and measured concentrations:
- 1000 mg/kg dry artificial soil weight (nominal)
- Reference substance (positive control):
- yes
- Remarks:
- chloracetamide (not determined during the test but in the same year 20 February to 6 March 2009)
Results and discussion
Effect concentrations
- Key result
- Duration:
- 14 d
- Dose descriptor:
- LC50
- Effect conc.:
- > 1 000 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Details on results:
- - Mortality at end of exposure period: no mortality- Total mass of adults at beginning of test: 310 to 330 mg- Total mass of adults at end of exposure period: 300 to 390 mg; Significant changes of the body weight (p>0.05) of the surviving earthworms were not observed at the end of the test- Morphological abnormalities: none- Behavioural abnormalities: none
- Results with reference substance (positive control):
- - Results with reference substance valid?: yesReference substance chloracetamide was tested at 4, 8, 16, 31, 60 mg/kg dry artificial soil. The 14 day-LC50 of a reference substance was determined to be 10 mg/kg dry soil weight (95% CL = 6 to 8 mg/kg dry soil weight) assuring that the laboratory test conditions were adequate.
Any other information on results incl. tables
Mortability of Eisenia foetida exposed to the test substance at 7 and 14 days
| Controls 0 mg/kg dry soil weight | Treatment 1000 mg/kg dry soil weight | ||
Replicates | 7 days | 14 days | 7 days | 14 days |
A | 0 | 0 | 0 | 0 |
B | 0 | 0 | 0 | 0 |
C | 0 | 0 | 0 | 0 |
D | 0 | 0 | 0 | 0 |
Total number of earthworms | 40 | 40 | 40 | 40 |
Number of death | 0 | 0 | 0 | 0 |
Mortality (%) | 0 | 0 | 0 | 0 |
No particular behavioral and morphological changes were observed.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- Based on the results, the 7 day and 14 day LC 50 was >1000 mg/kg dry soil weight
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