Registration Dossier

Data platform availability banner - registered substances factsheets

Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2009-08-07 to 2009-08-28
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2010
Report date:
2010

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to guideline
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Deviations:
no
Qualifier:
according to guideline
Guideline:
other: Guidelines for the Hazardous Evaluation of New Chemicals, HJ/T154-2004, China
GLP compliance:
yes

Test material

Constituent 1
Reference substance name:
2-Propyl-4-methyl-6-(1-methylbenzimidazole-2-yl)benzimidazole
IUPAC Name:
2-Propyl-4-methyl-6-(1-methylbenzimidazole-2-yl)benzimidazole
Test material form:
solid: particulate/powder
Remarks:
migrated information: powder
Details on test material:
- Storage condition of test material: storage room of the institute, preservation condition normal temperature

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
not specified

Test organisms

Test organisms (species):
other: Gobiocypris rarus
Details on test organisms:
TEST ORGANISM- Code of test fish: GR-IHB-090414- Source: Institute of Hydrobiology, Chinese Academy of Sciences on April 14, 2009- Age at study initiation (mean and range, SD): not specified- Length at study initiation (length definition, mean, range and SD): 2 to 3 cm (total length)- Weight at study initiation (mean and range, SD): 0.2 to 0.3 g- Feeding during test: noneACCLIMATION- Acclimation period: 4 month- Acclimation conditions (same as test or not): same- Feeding frequency: daily until 24 hours the test is started- Health during acclimation (any mortality observed): None, no diseases were observed during acclimation period. No mortality observed during 7 days period prior to initiation of test

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h

Test conditions

Hardness:
155 mg CaO3/L
Test temperature:
23.3 - 24.7 °C
pH:
6.75 - 7.84
Dissolved oxygen:
>90% of air saturation value (ASV)
Salinity:
not applicable
Nominal and measured concentrations:
nominal 60% and 80% saturated for measured concentrations refer to table 1
Details on test conditions:
TEST SYSTEM- Test vessel: 6L glass tanks- Aeration: yes- No. of organisms per vessel: 10- No. of vessels per concentration (replicates): 3- No. of vessels per control (replicates): 3- Biomass loading rate: ca 0.5 g Fish /LOTHER TEST CONDITIONS- Adjustment of pH: no- Photoperiod: 12 hours photoperiod daily, -feeding : none-oxigen concentration: >60% ASV-temperature: 23 +- 2°CEFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mortality, abnormal response, pH, dissolved Oxygen and water temperature, concentration of test substance at the begining and end of test by HPLCTEST CONCENTRATIONS- Range finding study: yes- Test concentrations: three concentrations : saturated concentration (1.150 mg/L measured at start, 1.332 mg/L at the end of the test (96h)), 20% (0.414 mg/L measured at start, 0.481 mg/L at the end of the test (96h)), 4 % (under the LOQ of HPLC) of the saturated concentration and one blank all with no repetitions- Results used to determine the conditions for the definitive study: yes
Reference substance (positive control):
no

Results and discussion

Effect concentrations
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 0.897 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Remarks on result:
other: 95% CL 0.88 to 0.99 mg/L
Details on results:
- Behavioural abnormalities: loss of equilibrium was observed in one fish at the test item treated group of saturated concentration - Mortality of control: yes

Any other information on results incl. tables

Sublethal observations / clinical signs:

Test results over a period of 96 h

Test group

Replicates

0

24

48

72

96

 

 

*a

*b

*a

*b

*a

*b

*a

*b

*a

*b

Blank

1

0

none

0

none

1

none

1

none

1

none

2

0

none

0

none

1

none

1

none

1

none

3

0

none

0

none

0

none

0

none

1

none

Saturated solution

1

0

none

0

none

4

none

4

none

5

none

2

0

none

0

none

4

none

4

none

4

none

3

0

none

0

none

4

none

4

none

4

none

80% saturated solution

1

0

none

0

none

0

none

0

none

0

none

2

0

none

0

none

0

none

0

none

0

none

3

0

none

0

none

2

none

2

none

2

none

60 % saturated solution

1

0

none

0

none

0

none

0

none

0

none

2

0

none

0

none

0

none

0

none

0

none

3

0

none

0

none

0

none

0

none

0

none

*a= cumulative number of dead fishes

*b= abnormal responses

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The 96-h LC50 value of > 0.897 mg/L +-10.69 µg/L (saturated concentration) to Gobiocypris rarus was determined for the test item under the conditions of the test.