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EC number: 213-138-3 | CAS number: 926-57-8
Toxicological information
Genetic toxicity: in vitro
Currently viewing:
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Adopted according to OECD SIDS (public available peer reviewed source). The original source is not available and has not been reviewed.
Data source
Referenceopen allclose all
- Reference Type:
- secondary source
- Title:
- 1,3-Dichlorobut-2-ene - CAS No: 926-57-8
- Author:
- OECD SIDS
- Year:
- 2 007
- Bibliographic source:
- SIDS Initial Assessment Report for 22th SIAM, UNEP Publications
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 980
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- yes
- Remarks:
- Only 4 strains tested
- Principles of method if other than guideline:
- The assay was performed in the presence and absence of a rat-liver homogenate activation system similar to the method described by Ames et al. (1975). Mutat. Res., 31:347-364.
- GLP compliance:
- no
- Remarks:
- GLP was not mandatory at the time of the study
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 1,3-dichlorobut-2-ene
- EC Number:
- 213-138-3
- EC Name:
- 1,3-dichlorobut-2-ene
- Cas Number:
- 926-57-8
- Molecular formula:
- C4H6Cl2
- IUPAC Name:
- 1,3-dichlorobut-2-ene
- Details on test material:
- - Name of test material (as cited in study report): 1,3-dichlorobut-2-ene
-Analytical purity: 99.5% (cis and trans)
Constituent 1
Method
- Target gene:
- his operone
Species / strain
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9 mix from rat
- Test concentrations with justification for top dose:
- 0, 10, 50, 100, 500, 1000, 2500 ug/plate (without metabolic activation); 0, 50, 100, 500, 1000, 2500 µg/plate (with metabolic activation)
- Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: DMSO
Controlsopen allclose all
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- Remarks:
- DMSO
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: 2-aminoanthracene
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- Remarks:
- DMSO
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- 9-aminoacridine
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- Remarks:
- DMSO
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- N-ethyl-N-nitro-N-nitrosoguanidine
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- Remarks:
- DMSO
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- 2-nitrofluorene
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- Remarks:
- DMSO
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: chloroethene
- Details on test system and experimental conditions:
- METHOD OF APPLICATION in agar (plate incorporation); preincubation; Spot Test in a Closed System; Plate Assay - Volatile Liquids
- Evaluation criteria:
- The test sample was classified as a non-mutagen when: 1) the probability was greater than 0.05 that the numbers of revertants at each of the test sample concentrations studied were not greater than the number of revertants in the solvent control and 2) the probability was greater than 0.05 that there was not a positive correlation between the numbers of revertants and increasing concentrations of the test substance.
The test substance was classified as a mutagen when: 1) the probability was less than 0.01 that the numbers of revertants at one or more of the test sample concentrations studied was not greater than the number of revertants in the solvent control, and 2) the probability was less than 0.01 that there was not a positive correlation between the number of revertants and increasing concentrations of the test substance. - Statistics:
- Two analyses were performed. In the first analysis, the response observed at each concentration was compared to the control by a t-test of significance. In the second analysis, the significance of the dose-response relationship was tested. Linear, quadratic, and higher order dose-response effects were tested in an F-test of significance. In addition, an analysis was conducted to determine whether the dose-response was different in different trials
Results and discussion
Test resultsopen allclose all
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with
- Genotoxicity:
- positive
- Remarks:
- TA100, plate incorporation assay
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- without
- Genotoxicity:
- positive
- Remarks:
- TA100, volatile liquid assay
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with
- Genotoxicity:
- positive
- Remarks:
- TA100 and TA1535, volatile liquid assay
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not specified
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- positive
- Remarks:
- TA 1535, without an activation system in trial 2 of the volatile liquid assay, but not in trial 1
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- not valid
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
positive
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