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Toxicological information

Acute Toxicity: inhalation

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Administrative data

Endpoint:
acute toxicity: inhalation
Type of information:
experimental study
Adequacy of study:
other information
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Adopted according to OECD SIDS. The original source is not available and has not been reviewed.

Data source

Referenceopen allclose all

Reference Type:
secondary source
Title:
1,3-Dichlorobut-2-ene - CAS No: 926-57-8
Author:
OECD SIDS
Year:
2007
Bibliographic source:
SIDS Initial Assessment Report for 22th SIAM, UNEP Publications
Reference Type:
publication
Title:
No information
Author:
Kwon, B. K. and R. S. Waritz (as cited in OECD SIDS)
Year:
1968
Bibliographic source:
Am. Ind. Hyg. Assoc. Meet

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
In order to determine the LC50 male rats were exposed for 4-hour to 1,3-dichloro-2-ene
GLP compliance:
no
Remarks:
GLP was not mandatory at the time of the study
Test type:
other: not specified
Limit test:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): 1,3-dichlorobut-2-ene
- Analytical purity: 92%

Test animals

Species:
rat
Strain:
other: ChR-CD
Sex:
male
Details on test animals and environmental conditions:
TEST ANIMALS
- Weight at study initiation: 250-286g

Administration / exposure

Route of administration:
inhalation: vapour
Type of inhalation exposure:
whole body
Vehicle:
air
Details on inhalation exposure:
The test material was metered into a glass U-tube immersed in a silicone oil bath at 120-125ºC. The resulting vapour was carried by a stream of air
into an 18-L glass chamber containing 10 rats.
Analytical verification of test atmosphere concentrations:
yes
Remarks:
The chamber atmospheric concentration was analyzed 8-10 times per exposure via gas chromotagraphy.
Duration of exposure:
4 h
Concentrations:
not specifed
No. of animals per sex per dose:
10
Control animals:
not specified
Details on study design:
- Duration of observation period following administration: 14 days
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight, histopathology
Statistics:
For calculation of the LC50, the Litchfield and Wilcoxon method was used on the 14-day mortality data.

Results and discussion

Effect levels
Sex:
not specified
Dose descriptor:
LC50
Effect level:
546 ppm
Based on:
test mat.
95% CL:
506 - 590
Exp. duration:
4 h
Mortality:
All deaths occurred post-exposure. Animals died between one day and 14 days post-exposure (no specific data presented for the individual isomers).
Clinical signs:
other: At lethal levels, the isomers caused irregular breathing, lacrimation, salivation, and hyperemia of the ears during exposure (no specific data presented on individual isomers).
Body weight:
Initial body weight loss by rats caused by all isomers was approximately 10%. Only 4/21 survivors regained their pre-exposure body weight within 14 days.
Gross pathology:
No data
Other findings:
- Histopathology: Histologic examination of tissues revealed lesions and hemorrhage of lungs, tracheitis, and cell degeneration in liver, spleen, thymus, and lymph nodes. Tubular degeneration of the kidneys was also noted.

Applicant's summary and conclusion