1,3-dichlorobut-2-ene

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

other information

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Adopted according to OECD SIDS. The original source is not available and has not been reviewed.

Data source

Reference

Reference 1

Reference Type:

secondary source

Title:

1,3-Dichlorobut-2-ene - CAS No: 926-57-8

Author:

OECD SIDS

Year:

2007

Bibliographic source:

SIDS Initial Assessment Report for 22th SIAM, UNEP Publications

Reference 2

Reference Type:

publication

Title:

No information

Author:

Kwon, B. K. and R. S. Waritz (as cited in OECD SIDS)

Year:

1968

Bibliographic source:

Am. Ind. Hyg. Assoc. Meet

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

In order to determine the LC50 male rats were exposed for 4-hour to 1,3-dichloro-2-ene

GLP compliance:

no

Remarks:

GLP was not mandatory at the time of the study

Test type:

other: not specified

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

1,3-dichlorobut-2-ene

EC Number:

213-138-3

EC Name:

1,3-dichlorobut-2-ene

Cas Number:

926-57-8

Molecular formula:

C4H6Cl2

IUPAC Name:

1,3-dichlorobut-2-ene

Details on test material:

- Name of test material (as cited in study report): 1,3-dichlorobut-2-ene
- Analytical purity: 92%

Test animals

Species:

rat

Strain:

other: ChR-CD

Sex:

male

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Weight at study initiation: 250-286g

Administration / exposure

Route of administration:

inhalation: vapour

Type of inhalation exposure:

whole body

Vehicle:

air

Details on inhalation exposure:

The test material was metered into a glass U-tube immersed in a silicone oil bath at 120-125ºC. The resulting vapour was carried by a stream of air
into an 18-L glass chamber containing 10 rats.

Analytical verification of test atmosphere concentrations:

yes

Remarks:

The chamber atmospheric concentration was analyzed 8-10 times per exposure via gas chromotagraphy.

Duration of exposure:

4 h

Concentrations:

not specifed

No. of animals per sex per dose:

10

Control animals:

not specified

Details on study design:

- Duration of observation period following administration: 14 days
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight, histopathology

Statistics:

For calculation of the LC50, the Litchfield and Wilcoxon method was used on the 14-day mortality data.

Results and discussion

Effect levels

Sex:

not specified

Dose descriptor:

LC50

Effect level:

546 ppm

Based on:

test mat.

95% CL:

506 - 590

Exp. duration:

4 h

Mortality:

All deaths occurred post-exposure. Animals died between one day and 14 days post-exposure (no specific data presented for the individual isomers).

Clinical signs:

other: At lethal levels, the isomers caused irregular breathing, lacrimation, salivation, and hyperemia of the ears during exposure (no specific data presented on individual isomers).

Body weight:

Initial body weight loss by rats caused by all isomers was approximately 10%. Only 4/21 survivors regained their pre-exposure body weight within 14 days.

Gross pathology:

No data

Other findings:

- Histopathology: Histologic examination of tissues revealed lesions and hemorrhage of lungs, tracheitis, and cell degeneration in liver, spleen, thymus, and lymph nodes. Tubular degeneration of the kidneys was also noted.