Registration Dossier

Environmental fate & pathways

Biodegradation in water: screening tests

Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2016
Report Date:
2016

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
GLP compliance:
yes (incl. certificate)

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
solid: crystalline
Specific details on test material used for the study:
Manufacturer: Anhui Shindo Chemical CO. LTDSupplier: Changzou Foreign Trade Corp.Batch number: 0102A26 (20151203)Physical state: Solid, crystalline substanceColour: WhiteStorage: in a cool, dry place, protected from light

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, non-adapted
Details on inoculum:
Type of inoculum: aerobic mixed bacterial cultureSource: Municipal wastewater treatment plant, Esztergom-Kertváros, Hungary
Duration of test (contact time):
28 d
Parameter followed for biodegradation estimation
Parameter followed for biodegradation estimation:
O2 consumption
Details on study design:
PREPARATION OF INOCULUM TEST CULTUREBefore the test 300 ml stock culture was filtered through a filter with 0.2 mm pore size to remove the bigger insoluble particles and then the dry substance content of the filtrate was determined. The measured value was 3.84 g/L. For preparing the inoculum culture 147.92 ml mineral medium was added to 52.1 ml filtered stock culture. The calculated dry mass of inoculum culture was 1 g/L.PREPARATION OF REFERENCE SOLUTIONFor the reference solution 320.02 mg sodium acetate was diluted in 100 ml mineral medium.PREPARATION OF TEST VESSELSFollowing test vessels were prepared and analysed for the study:- Test item (in two replicates)- Blank control (in two replicates)- Reference solution (positive control)- Reference and test item solution (toxicity control)- Sterilized solution of test item (abiotic control)The vessels were kept in thermostat for 28 days.
Reference substance
Reference substance:
acetic acid, sodium salt

Results and discussion

% Degradation
Parameter:
% degradation (O2 consumption)
Value:
2
Remarks on result:
other: mean of two replicates

BOD5 / COD results

Results with reference substance:
Reference solution (positive control): 75%

Applicant's summary and conclusion

Interpretation of results:
not readily biodegradable
Conclusions:
According to OECD guideline the test item should be classified as readily biodegradable if the percentage biodegradation reaches 60% of theoretical oxygen demand within 28 day period in a 10-day window. Based on ready biodegradability testing results the Amidinothiourea is not readily biodegradable.