Registration Dossier

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2016
Report Date:
2016

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
yes (incl. certificate)

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
solid: crystalline
Specific details on test material used for the study:
Manufacturer: Anhui Shindo Chemical CO. LTDSupplier: Changzou Foreign Trade Corp.Batch number: 0102A26 (20151203)Physical state: Solid, crystalline substanceColour: WhiteStorage: in a cool, dry place, protected from light

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
yes
Details on test solutions:
Seven dilution levels were prepared by mixing appropriate volume of Elendt M4 medium and stock solution of the test item. The maximum separation factor between of test vessels was 2.0. The volume of the prepared test solutions was 300 ml.

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
Test species: Daphnia magna StrausAge: < 24 hours neonatesOrigin: National Institute of Environmental HealthCulture conditions: The test species is maintained in Elendt M4 medium with culture conditions at 21±1 °C and a light/dark cycle of 16/8 hours with light intensities of less than 1000 Lux. The cultures are fed with centrifugalized green alga (Pseudokirchneriella subcapitata) cells daily at weekdays.

Study design

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h
Post exposure observation period:
The number of immobilised and abnormally behaved daphnids was recorded after 24 and 48h.

Test conditions

Test temperature:
The temperature of thermostat was recorded daily and registered continuously by Extech datalogger.Temperature at the start of the test: 21.2 °CTemperature after 24 hours: 21.0 °CTemperature at the end of the test: 21.0 °CThe temperature inside the thermostat at the test vessels was measured and registered continuously by Extech SD200 thermometer and datalogger.
pH:
The pH was measured at the beginning and at the end of the test. At the beginning of the test the pH was measured from the remaining test solutions of the concurrent test vessels at each dilution levels. At the end of the test the pH was measured in each test vessel. There was not any significant change in the pH during the test.
Dissolved oxygen:
The dissolved oxygen was measured at the beginning and at the end of the test. At the beginning of the test the dissolved oxygen was measured from theremaining test solutions of the concurrent test vessels at each dilution levels. At the end of the test the dissolved oxygen was measured in each test vessel. There was not any significant change in the oxygen concentration during the test.
Nominal and measured concentrations:
In the test the following nominal concentrations of test solutions were applied: 100; 50; 40; 30; 20; 15 and 10 mg/L. The following actual concentrations were calculated from the measured values in the test: 99.05; 49.31; 40.37; 29.88; 19.63; 14.40 and 9.11 mg test item/L.
Details on test conditions:
The test was maintained under static conditions for a period of 48 hours. At the start of the study each test vessels contained 50 ml of test solutions and five young daphnids. In the test four replicates total of 20 animals at each test concentration level were investigated. The test vessels were covered with plastic lids and were kept in thermostat at 21 ± 1 °C with a 16/8 hours light/dark cycle. Each control vessels containing five young daphnids in Elendt M4 medium were prepared in four replicates and kept under the same static conditions. The number of immobilised and abnormally behaved daphnids was recorded after 24 and 48h. A daphnia is considered to be immobile if it is not able to swim within 15 seconds after a gentle agitation of the test vessel. The daphnids were not fed during the test.

Results and discussion

Effect concentrationsopen allclose all
Duration:
48 h
Dose descriptor:
EC10
Effect conc.:
19.64 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
mobility
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
30.25 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
The ECx concentrations were determined by the ToxRat® statistic software.

Applicant's summary and conclusion

Conclusions:
In this Daphnia sp., Acute Immobilisation Test for Amidinothiourea the results indicated that the test item is hazardous to the aquatic environment and classified into the third acute toxic hazardous category [GHS Acute aquatic hazard Category Acute III. – 48 hr EC50 (for Crustacea) > 10 mg/L but ≤ 100 mg/L].