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Environmental fate & pathways

Biodegradation in water and sediment: simulation tests

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Administrative data

Endpoint:
biodegradation in water and sediment: simulation tests
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Endpoint study record was created to contribute to the overall results; acceptable, well documented publication/study report which meets basic scientific principles

Data source

Referenceopen allclose all

Reference Type:
publication
Title:
Bildung von Chlorphenolen durch mikrobielle Umwandlung von Chlorbenzolen
Author:
Ballschmiter K, C Unglert und P Heizmann
Year:
1977
Bibliographic source:
Angew. Chem 89(9):680-681
Reference Type:
secondary source
Title:
m-dichlorobenzene BUA-Stoffbericht 8 (Juni 1981) [Report in German]
Author:
GDCh, BUA
Year:
1987
Bibliographic source:
publisher: VCH; ISSN: 0179-2601

Materials and methods

Principles of method if other than guideline:
other: biotransformation using mixed and pure cultures of bacteria
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
1,3-dichlorobenzene
EC Number:
208-792-1
EC Name:
1,3-dichlorobenzene
Cas Number:
541-73-1
Molecular formula:
C6H4Cl2
IUPAC Name:
1,3-dichlorobenzene
Test material form:
other: liquid
Details on test material:
no data

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
other: precultured mixed cultures of soil bacteria
Details on inoculum:
Were used to benzene precultured mixed cultures of soil bacteria, preferably gram-negative polar flagellated rods, which still stood in the mineral salt solution (pH = 6.8) benzene as a carbon source.
Duration of test (contact time):
24 - 500 h
Initial test substance concentration
Initial conc.:
20 - 200 µg/L
Based on:
test mat.
Details on study design:
200 mg of benzene and 50 mg of 1,3-dichlorobenzne diffused layer of a hard paraffin (2 g) in 200 mL culture medium.
Here, a 0.000001 and 0.0000001 M solution of 1,3-dichlorbenzene (about 20 to 200 g / L) was established
After 24-, 100-, 240- and 500 hours incubation at 28 ° C were worked together on chlorophenols cells and nutrient medium.

Results and discussion

Transformation products:
yes
Identity of transformation productsopen allclose all
No.:
#1
Reference
Reference substance name:
Unnamed
IUPAC name:
2,4-dichlorophenol
Inventory number:
InventoryMultipleMappingImpl [inventoryEntryValue=EC 204-429-6]
CAS number:
120-83-2
Identity:
Phenol, 2,4-dichloro-
Molecular formula:
C6H4Cl2O
Molecular weight:
163.001
SMILES notation:
Oc1ccc(Cl)cc1Cl
InChl:
InChI=1/C6H4Cl2O/c7-4-1-2-6(9)5(8)3-4/h1-3,9H
No.:
#2
Reference
Reference substance name:
Unnamed
IUPAC name:
2,5-dichlorophenol
Inventory number:
InventoryMultipleMappingImpl [inventoryEntryValue=EC 209-520-4]
CAS number:
583-78-8
Identity:
2,5-dichlorophenol
Identity:
Phenol, 2,5-dichloro-
Molecular formula:
C6H4Cl2O
Molecular weight:
163.001
SMILES notation:
Oc1cc(Cl)ccc1Cl
InChl:
InChI=1/C6H4Cl2O/c7-4-1-2-5(8)6(9)3-4/h1-3,9H
No.:
#3
Reference
Reference substance name:
Unnamed
IUPAC name:
2,6-dichlorophenol
Inventory number:
InventoryMultipleMappingImpl [inventoryEntryValue=EC 201-761-3]
CAS number:
87-65-0
Identity:
2,6-dichlorophenol
Identity:
Phenol, 2,6-dichloro-
Molecular formula:
C6H4Cl2O
Molecular weight:
163.001
SMILES notation:
Oc1c(Cl)cccc1Cl
InChl:
InChI=1/C6H4Cl2O/c7-4-2-1-3-5(8)6(4)9/h1-3,9H

Any other information on results incl. tables

The elimination of 1,3-dichlorobenzene through adsorption on activated carbon and biodegradation by film-bacteria resulted in an elimination rate of 95 ± 2 % in a column with continuous flow over two years.

In the control (only film-bacteria) was the reduction only 28 ± 32 % after adaptation.

A final statement on the biodegradation of 1,3-dichlorobenzene based on these results is not possible.

The biodegradation of chlorobenzene to chlorophenol by soil bacteria in aquatic solutions is due to the action of a monooxygenase.

Applicant's summary and conclusion

Validity criteria
Validity criteria fulfilled:
not specified
Conclusions:
The elimination of 1,3-dichlorobenzene through adsorption on activated carbon and biodegradation by film-bacteria resulted in an elimination rate of 95 ± 2 % in a column with continuous flow over two years.
In the control (only film-bacteria) was the reduction only 28 ± 32 % after adaptation.
A final statement on the biodegradation of 1,3-dichlorobenzene based on these results is not possible.
The biodegradation of chlorobenzene to chlorophenol by soil bacteria in aquatic solutions is due to the action of a monooxygenase.
Executive summary:

The elimination of 1,3-dichlorobenzene through adsorption on activated carbon and biodegradation by film-bacteria resulted in an elimination rate of 95 ± 2 %in a column with continuous flow over two years.

In the control (only film-bacteria) was the reduction only 28 ± 32 % afteradaptation.

A final statement on the biodegradation of 1,3-dichlorobenzene based on these results is not possible.

The biodegradation of chlorobenzene to chlorophenol by soil bacteria in aquatic solutions is due to the action of a monooxygenase.

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