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Environmental fate & pathways

Bioaccumulation: aquatic / sediment

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Administrative data

Endpoint:
bioaccumulation in aquatic species: fish
Type of information:
experimental study
Adequacy of study:
other information
Study period:
1982-07-05 till 1983-09-07
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Guideline study with acceptable restrictions.

Data source

Reference
Reference Type:
publication
Title:
Unnamed
Year:
1992

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 305 C (Bioaccumulation: Test for the Degree of Bioconcentration in Fish)
Deviations:
no
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
1,3-dichlorobenzene
EC Number:
208-792-1
EC Name:
1,3-dichlorobenzene
Cas Number:
541-73-1
Molecular formula:
C6H4Cl2
IUPAC Name:
1,3-dichlorobenzene
Test material form:
other: liquid
Details on test material:
- Purity: 98 % (according from the sponsor)
- Empirical formula: C6H4Cl2
- Molecular mass: 147.0 g/mole

Sampling and analysis

Details on sampling:
- Concentrations: The first was 100 µg/L and the second was 10 µg/L of 1,3-dichlorobenzene.
The test water analysis was perfomed twice a week (n=1).
The fish analysis was performed every two weeks (n=2).
The control fish analysis was performed before the initiation and the termination of exposure.

Test solutions

Vehicle:
yes
Details on preparation of test solutions, spiked fish food or sediment:
Farming conditions:
- To remove some of the abnormal by the visual observation when fish availability, Acceptance tank in medicine Yokugo, I was 1-2 months bred in running water state.
- Desinfection: For external disinfection, the fish were placed in an aqueous solution (7 g/L sodium chloride) in a water-stop state for 24
hours.
- Acclimation: at 25 ° C for 14 days
- Stock solution: 0.1 g of 1,3-dichlorobenzene was dissolved in n-hexane to 100 mL (1000 mg/L)

- Chemical name of vehicle (organic solvent, emulsifier or dispersant): Tween-80 (Polyoxyethylensorbitanmonooleat; 48 h LC50 = 500000 mg/L)
- Concentration of vehicle in test medium: 1000 mg/L


Test organisms

Test organisms (species):
Cyprinus carpio
Details on test organisms:
- Name: Carp (Cyprinus carpio)
- Supplier: Sugishima fish farm
- Conditions for fish keeping: Period etc.
At reception, fish showing visible abnormal signs were removed. Then fish were reared according to flow through system for 1-2 months after external
disinfection.
- External disinfection
External disinfection was performed according to static condition for 24 hours in an aqueous solution containing 50 mg/L Terramycin (Taito Pfizer) and 7 g/L sodium chloride.
- Condition of acclimation: Fish were reared in an acclimation tank according to flow through system at temperature of 25 + 2 °C for about 28 days. During the period, abnormal fish were removed. Then the fish were transfered to test tanks and reared again at the same temperature according to flow through system for about one month.
- Weight, length and lipid content.at the initiation of exposure: Weight = 32.2 g, Length = 10.7 cm, Lipid content = 4.8 %
- Feeding:
Feed = Pelleted feed for carp
Manufacturer = Japan Haigo Shiryo K.K.
Feeding amount and interval. Amount corresponding to about 2 % of the total body weight of test fish was fed twice a day by halves.
On the day of fish sampling feed.was not supplied.

Study design

Route of exposure:
aqueous
Test type:
flow-through
Water / sediment media type:
natural water: freshwater
Total exposure / uptake duration:
8 wk

Test conditions

Hardness:
not specified
Test temperature:
25 °C
pH:
not specified
Dissolved oxygen:
6-8 mg/L
Details on test conditions:
- Temperature: 25 ± 2 °C; Temperature controlled test water was supplied continuously into the flow through system (Aquatron).
- Test tank: Glass tank of 100 liters in volume, exchange frequency 1-2 times per month
- Flow rate of test water: At a rate of 800 L/day of test water was supplied.
- Number of fish (at the initiation of exposure): 25 fish/Glass tank
- During the exposure: 8 weeks
- Feeding: Food was shared, 2 times per day, 2 % of fish body weight
- Photoperiod: 14 h light / 10 h dark

Nominal and measured concentrations:
nominal concentrations: first = 100 µg/L and second = 10 µg/L
Reference substance (positive control):
no
Details on estimation of bioconcentration:
BASIS INFORMATION
- Measured logPow: 3.72

BASIS FOR CALCULATION OF BCF
BCF = concentration of test substance in fish/concentration of test substance in water

Results and discussion

Lipid content
Lipid content:
4.8 %
Time point:
end of exposure
Bioaccumulation factoropen allclose all
Type:
BCF
Value:
58 - 370
Remarks on result:
other: Conc.in environment / dose:10 µg/L
Type:
BCF
Value:
57 - 229
Remarks on result:
other: Conc.in environment / dose:100 µg/L

Applicant's summary and conclusion

Validity criteria fulfilled:
not specified
Remarks:
(expected to be a valid source)
Conclusions:
The experimental BCF values in carp (Cyprinus carpio) after 8 weeks of exposure are 57 - 229 (concentration 100 µg/L) and 58 - 370
(concentration 10 µg/L) for 1,3-dichlorobenzene. 1,3-dichlorobenzene should be considered to "Not Bioaccumulative in Aquatic Organisms" according to the EC regulation no 1272/2008.
Executive summary:

The determination of the bioconcentration of 1,3-dichlorobenzene was tested according to the guideline "Bioaccumulation test of chemical substances in fish and shellfish" (1974) and corresponding to OECD guideline 305C (Bioaccumulation: Test for the degree of bioconcentration in Fish, 1981). They exposed Cyprinus carpio (carp) with a lipid content of 4.8 % to 100 µg/L and 10 µg/L of 1,3 -dichlorobenzene during 8 weeks. After this period they obtained experimental BCF values of 57 - 229 (concentration 100 µg/L) and 58 - 370 (concentration 10 µg/L). 1,3 -dichlorobenzene should be considered to "Not Bioaccumulative in Aquatic Organisms" according to the EC regulation no 1272/2008.

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