Registration Dossier

Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: The test was carried out according to German national standards, however no information was given on the purity of the test substance.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1989
Report Date:
1989

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
other: German standard DIN 38412 L9
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): D-Pantolactone
Purity: no data
Water solubility: 950 g/l

Sampling and analysis

Analytical monitoring:
no

Test solutions

Details on test solutions:
Each test concentration was prepared separately.

Test organisms

Test organisms (species):
Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
Details on test organisms:
TEST ORGANISM
- Strain:SAG 86.81
- Source (laboratory, culture collection): Algae collection Goettingen, Germany

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
72 h

Test conditions

Test temperature:
21.4 °C
pH:
7.75 - 7.99
Nominal and measured concentrations:
Nominal test concentrations: 0 (control), 25, 50, 100, 250, and 500 mg/l
Details on test conditions:
TEST SYSTEM
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- No. of vessels per vehicle control (replicates): 4



TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according to DIN 38412 L9



EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: fluormetrically


Reference substance (positive control):
yes
Remarks:
Potassium chromate p.a.

Results and discussion

Effect concentrationsopen allclose all
Duration:
72 h
Dose descriptor:
EC10
Effect conc.:
> 500 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
> 500 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Reported statistics and error estimates:
Statistics were performed according to Tallarida RJ and Jacob LS, The dose-response relation in pharmacology, 98 -103, Springer

Any other information on results incl. tables

ECx values (in mg/l) after 72 hours of exposure

ErC10 > 500 mg/l
ErC50 > 500 mg/l

EbC10 = 211.61 mg/l
EbC50 > 500 mg/l

Applicant's summary and conclusion