Registration Dossier

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: guideline study, scientifically accepable however no information was given on the purity of the test substance.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1989
Report Date:
1989

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
other: German standard DIN 38412 L 11.
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): D-Pantolactone (crystalline)
Purity: no data
Water solubility: 950 g/l

Sampling and analysis

Analytical monitoring:
no

Test solutions

Details on test solutions:
The stock solution of nominal 2000 mg/l in a culture medium was prepared. Following this, serial dilutions of this solution were prepared in order to obtain the desired test concentrations.

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name:
- Strain:
- Source: Daphnia magna Straus, originally obtained from the Bundesgesundheitsamt, Berlin.
- Age at study initiation (mean and range, SD): 2 - 24 hours
- Weight at study initiation (mean and range, SD):
- Length at study initiation (length definition, mean, range and SD):
- Valve height at study initiation, for shell deposition study (mean and range, SD):
- Peripheral shell growth removed prior to test initiation:
- Method of breeding:
- Feeding during test
- Food type:
- Amount:
- Frequency:


ACCLIMATION
- Acclimation period:
- Acclimation conditions (same as test or not):
- Type and amount of food: 1 x daily grown Green algae culture (Scenedesmus subspicatus)
- Feeding frequency:
- Health during acclimation (any mortality observed):


QUARANTINE (wild caught)
- Duration:
- Health/mortality:

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h

Test conditions

Test temperature:
21 °C
pH:
7.2 - 7.7
Dissolved oxygen:
7.9 - 8.2 mg/l
Nominal and measured concentrations:
Nominal test concentrations: 18, 32, 58, 100, 180, 320, 580 mg/l. Additionally, a control was tested in parallel.
Details on test conditions:
TEST SYSTEM
- Test vessel: glass beaker
- Fill volume: 20 ml
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: chemistry: according to the test guideline DIN 38412, L 11
- Na /K ration: 20:1
- Ca/mg ratio: 5:1
- Conductivity: 658 µS/cm



OTHER TEST CONDITIONS
- Light intensity: 10 [µE/qm * s]


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Mobility after 0, 3, 6, 24, 48 h

Results and discussion

Effect concentrationsopen allclose all
Duration:
48 h
Dose descriptor:
EC0
Effect conc.:
> 580 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 580 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC100
Effect conc.:
> 580 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility

Any other information on results incl. tables

Cumulative immobilization of daphnia after x hours [%]: 
Nominal test concentration (mg/l)
/ 3 / 6 / 24 / 48 /
Control / 0 / 0 / 0 / 0 /
18 / 0 / 0 / 0 / 15 /
32 / 0 / 0 / 0 / 20 /
58 / 0 / 0 / 0 / 0 /
100 / 5 / 5 / 5 / 5 /
320 / 0 / 0 / 0 / 0 /
580 / 0 / 0 / 0 / 0 /

Applicant's summary and conclusion