Registration Dossier
Registration Dossier
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EC number: 943-540-0 | CAS number: -
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
Description of key information
The acute toxicty of the test item to the freshwater fish rainbow trout (Oncorhynchus mykiss) was investigated using the threshold approach and gave a 96 hour LL50 value of greater than 0.75 mg/L loading rate WAF. The No Observed Effect Loading rate was 0.75 mg/L loading rate WAF.
Key value for chemical safety assessment
Additional information
GUIDELINE
A study was performed to assess the acute toxicity of the test item to rainbow trout (Oncorhynchus mykiss). The method was designed to be compatible with the OECD Guidelines for Testing of Chemicals (1992) No 203 "Fish, Acute Toxicity Tes" referenced as Method C.1 of Commission Regulation (EC) No. 440/2008.
METHODS
Due to the low aqueous solubility and complex nature of the test item, the test medium was prepared as a Water Accommodated Fraction (WAF).
In accordance with the recommendations of REACH, the test was conducted according to the threshold approach recommended by ECHA. Using this approach, the lowest EL50 value from either the algal growth inhibition study or acute toxicity to Daphnia magna study is set as the threshold loading rate and a "limit test" is conducted at this threshold loading rate. If no mortalities are observed this indicates that fish are not the most sensitive species and that the LL50 is greater than the threshold loading rate. The EL 50 value obtained from the algal growth inhibition study was the lowest of the two EL50 values and hence the test was conducted at a single loading rate of 0.75 mg/L.
Seven fish were exposed to a Water Accommodated Fraction (WAF) of the test item, at a single nominal loading rate of 0.75 mg/L for a period of 96 hours at a temperature of 14°C to 15°C under semi-static test conditions. The number of mortalities and any sublethal effects of exposure in each test and control vessel were determined 3 and 6 hours after the start of exposure and then daily throughout the test until termination after 96 hours.
RESULTS
Analysis of the freshly prepared test media at zero and 72 hours showed that measured concentrations of 0.0682 and 0.138 mg/L were obtained respectively. Analysis of old test media at 24 and 96 hours gave measured concentrations of 0.0616 and 0.120 mg/L respectively.
Given that the toxicity cannot be attributed to a single component or a mixture of components, but to the test item as a whole, the results were based on nominal loading rates only.
CONCLUSION
The acute toxicty of the test item to the freshwater fish rainbow trout (Oncorhynchus mykiss) was investigated using the threshold approach and gave a 96 hour LL50 value of greater than 0.75 mg/L loading rate WAF. The No Observed Effect Loading rate was 0.75 mg/L loading rate WAF.
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