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Ecotoxicological information

Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study
Justification for type of information:
Experimental test result performed according to the guideline.
Qualifier:
according to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Principles of method if other than guideline:
Study was conducted to access the effect of test chemical 3-methoxyacetophenone on the growth of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test).
GLP compliance:
not specified
Analytical monitoring:
not required
Remarks:
According to test (If substance is stable and if not analytical done by spectrophotometer)
Vehicle:
not specified
Details on test solutions:
The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 400 mg of the test substance in 4 liters of potable water (passed through reverse osmosis system) with continuous stirring. This test solution was then added to the remaining three liters of water for achieving test concentrations of 100 mg/L.
Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
TEST ORGANISM
- Common name:Zebra Fish
- Strain:Danio rerio
- Source: Unique Aqua Fish Corner
- Length at study initiation (length definition, mean, range and SD): average ----- cm
- Weight at study initiation (mean and range, SD): average ------- g
-Housing:The fishes were kept in a static tank in tap water passed through reverse osmosis system, under natural conditions along with proper feed and aeration.
-Duration of housing: 15 days
-Photoperiod: ----------
-Diet : Standard brand feed, once daily
-Acclimatization: seven days
-feeding frequency: Once in a day
Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
96 h
Hardness:
150.5 mg of CaCO3
Test temperature:
24.4 °C
pH:
7.7
Dissolved oxygen:
7.7 mg/l
Nominal and measured concentrations:
100 mg/l
Details on test conditions:
TEST SYSTEM
- Test vessel: Plastic aquaria
- Material, size, headspace, fill volume: 5 liters of plastic aquaria filled with 2 liter of water
- Aeration: Aeration in test vessels was provided 1 day before the start of experiment
- No. of organisms per vessel: 8 fishes

OTHER TEST CONDITIONS
- Photoperiod: 16 hours light and 8 hours dark

Reference substance (positive control):
not specified
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other:

 

Nominal

Concentration

(mg/l)

Number of

fish

pH readings after

24 hr

48hr

72hr

96hr

V1

V2

V1

V2

V1

V2

V1

V2

Control

4

4

7.5

7.5

8.0

8.1

8.0

8.0

8.1

8.1

100

4

4

7.7

7.7

8.0

8.0

8.31

8.32

8.1

8.1

 

Nominal

Concentration

(mg/l)

Number of

fish

Oxygen content (mg/l) after

24 hr

48hr

72hr

96hr

V1

V2

V1

V2

V1

V2

V1

V2

Control

4

4

6.0

6.0

5.9

5.9

5.9

5.9

5.8

5.8

100

4

4

5.9

5.9

5.8

5.8

5.8

5.8

5.7

5.7

 

Temperature:

Nominal

Concentration

(mg/l)

Number of

fish

Temperature (°C) after

24 hr

48hr

72hr

96hr

V1

V2

V1

V2

V1

V2

V1

V2

Control

4

4

23.9

23.9

22.7

22.7

22.3

22.3

22.4

22.4

100

4

4

23.8

23.8

22.3

22.3

22.7

22.7

22.6

22.6

 

Validity criteria fulfilled:
yes
Conclusions:
Based on nominal concentrations, experimental median lethal Concentrations [LC-50 (96 h)] for CAS No. 586-37-8 on Zebra Fish Danio rerio was determine to be 100 mg/l.
Executive summary:

Study was conducted to access the effect of test chemical 3-methoxyacetophenone on the growth of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test). The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 400 mg of the test substance in 4 liters of potable water (passed through reverse osmosis system) with continuous stirring. This test solution was then added to the remaining three liters of water for achieving test concentrations of 100 mg/L and Zebra FishDanio reriowere exposed to these concentration for 96 hours. Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. After 96 hours of exposure to test item ( 3-methoxyacetophenone ) to nominal test concentration, LC50 was determine to be 100 mg/l . Based on the LC50, it can be consider that the chemical was not hazardous and can be consider to be not classified as per the CLP classification criteria.

Description of key information

After 96 hours of exposure to test item ( 3-methoxyacetophenone ) to nominal test concentration, LC50 was determine to be 100 mg/l . Based on the LC50, it can be consider that the chemical was not hazardous and can be consider to be not classified as per the CLP classification criteria.

Key value for chemical safety assessment

LC50 for freshwater fish:
100 mg/L

Additional information

Study was conducted to access the effect of test chemical 3-methoxyacetophenone on the growth of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test). The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 400 mg of the test substance in 4 liters of potable water (passed through reverse osmosis system) with continuous stirring. This test solution was then added to the remaining three liters of water for achieving test concentrations of 100 mg/L and Zebra FishDanio reriowere exposed to these concentration for 96 hours. Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. After 96 hours of exposure to test item ( 3-methoxyacetophenone ) to nominal test concentration, LC50 was determine to be 100 mg/l . Based on the LC50, it can be consider that the chemical was not hazardous and can be consider to be not classified as per the CLP classification criteria.