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Toxicological information

Genetic toxicity: in vitro

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Administrative data

Endpoint:
in vitro gene mutation study in bacteria
Remarks:
Type of genotoxicity: gene mutation
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Data is from peer reviewed publication

Data source

Reference
Reference Type:
publication
Title:
Mutagenicity Produced by Aqueous Chlorination of Organic Compounds
Author:
W. Howard Rapson, Mark A. Nazar, and Victor V. Butsky
Year:
1980
Bibliographic source:
Bull. Environm. Contam. Toxicol, 24,590-596 (1980)

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
other: as mentioned below
Principles of method if other than guideline:
Ames assay was performed to evaluated the mutagenic nature of the test compound m-Methoxyacetophenone
GLP compliance:
not specified
Type of assay:
bacterial gene mutation assay

Test material

Reference
Name:
Unnamed
Type:
Constituent
Type:
Constituent
Test material form:
other: Liquid
Details on test material:
- Name of test material (as cited in study report): m-Methoxyacetophenone
- Molecular formula (if other than submission substance): C10H12O2
- Molecular weight (if other than submission substance): 164.2028 g/mol
- Substance type: Organic
- Physical state: No data available
Purity No data available
- Impurities (identity and concentrations): No data available

Method

Species / strain
Species / strain / cell type:
S. typhimurium TA 100
Details on mammalian cell type (if applicable):
No data available
Additional strain / cell type characteristics:
not specified
Metabolic activation:
not specified
Metabolic activation system:
No data
Test concentrations with justification for top dose:
103, 102, 101, 100 and 10-1 µg per plate.
Vehicle / solvent:
No data available
Controls
Untreated negative controls:
not specified
Negative solvent / vehicle controls:
not specified
True negative controls:
not specified
Positive controls:
not specified
Positive control substance:
not specified
Details on test system and experimental conditions:
METHOD OF APPLICATION: in agar (plate incorporation)

DURATION
- Preincubation period: No data available
- Exposure duration: No data available
- Expression time (cells in growth medium): No data available
- Selection time (if incubation with a selection agent): No data available
- Fixation time (start of exposure up to fixation or harvest of cells): No data available

SELECTION AGENT (mutation assays): No data available
SPINDLE INHIBITOR (cytogenetic assays): No data available
STAIN (for cytogenetic assays): No data available

NUMBER OF REPLICATIONS: No replications were performed

NUMBER OF CELLS EVALUATED: No data available

DETERMINATION OF CYTOTOXICITY
- Method: mitotic index; cloning efficiency; relative total growth; other: No data available

OTHER EXAMINATIONS:
- Determination of polyploidy: No data available
- Determination of endoreplication: No data available
- Other: No data available

OTHER: No data available
Evaluation criteria:
Increase in the number of revertants, Toxicity toward the Ames Salmonella TA100 strain used was roughly estimated by visual comparison of the background lawn on the plate under a microscope, with 100% representing the same number of bacteria per unit area as the blank with no test solution (no toxicity) and 0 representing death of all bacteria.
Statistics:
No data available

Results and discussion

Test results
Species / strain:
S. typhimurium TA 100
Metabolic activation:
not specified
Genotoxicity:
negative
Cytotoxicity / choice of top concentrations:
not specified
Vehicle controls validity:
not specified
Untreated negative controls validity:
not specified
Positive controls validity:
not specified
Remarks on result:
other: strain/cell type:
Remarks:
Migrated from field 'Test system'.

Applicant's summary and conclusion

Conclusions:
Interpretation of results (migrated information):
negative

The test compound failed to induce mutation in the Salmonella typhimurium TA100 and hence is not a mutagen.
Executive summary:

Ames assay was performed to evaluate the mutagenic nature of the test compound m-Methoxyacetophenone usingSalmonella typhimuriumTA100. The test compound was tested at dose levels of 103, 102, 101, 100and 10-1ug per plate.

 

The test compoundm-Methoxyacetophenonefailed to induce mutation in theSalmonella typhimuriumTA100 and hence is not a mutagen.