Registration Dossier
Registration Dossier
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EC number: 209-573-3 | CAS number: 586-37-8
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Data is from peer reviewed publication
Data source
Reference
- Reference Type:
- publication
- Title:
- Mutagenicity Produced by Aqueous Chlorination of Organic Compounds
- Author:
- W. Howard Rapson, Mark A. Nazar, and Victor V. Butsky
- Year:
- 1 980
- Bibliographic source:
- Bull. Environm. Contam. Toxicol, 24,590-596 (1980)
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: as mentioned below
- Principles of method if other than guideline:
- Ames assay was performed to evaluated the mutagenic nature of the test compound m-Methoxyacetophenone
- GLP compliance:
- not specified
- Type of assay:
- bacterial gene mutation assay
Test material
- Reference substance name:
- 3-methoxyacetophenone
- EC Number:
- 209-573-3
- EC Name:
- 3-methoxyacetophenone
- Cas Number:
- 586-37-8
- Molecular formula:
- C9H10O2
- IUPAC Name:
- 1-(3-methoxyphenyl)ethan-1-one
- Reference substance name:
- m-Methoxyacetophenone
- IUPAC Name:
- m-Methoxyacetophenone
- Test material form:
- other: Liquid
- Details on test material:
- - Name of test material (as cited in study report): m-Methoxyacetophenone
- Molecular formula (if other than submission substance): C10H12O2
- Molecular weight (if other than submission substance): 164.2028 g/mol
- Substance type: Organic
- Physical state: No data available
Purity No data available
- Impurities (identity and concentrations): No data available
Constituent 1
Constituent 2
Method
Species / strain
- Species / strain / cell type:
- S. typhimurium TA 100
- Details on mammalian cell type (if applicable):
- No data available
- Additional strain / cell type characteristics:
- not specified
- Metabolic activation:
- not specified
- Metabolic activation system:
- No data
- Test concentrations with justification for top dose:
- 103, 102, 101, 100 and 10-1 µg per plate.
- Vehicle / solvent:
- No data available
Controls
- Untreated negative controls:
- not specified
- Negative solvent / vehicle controls:
- not specified
- True negative controls:
- not specified
- Positive controls:
- not specified
- Positive control substance:
- not specified
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: in agar (plate incorporation)
DURATION
- Preincubation period: No data available
- Exposure duration: No data available
- Expression time (cells in growth medium): No data available
- Selection time (if incubation with a selection agent): No data available
- Fixation time (start of exposure up to fixation or harvest of cells): No data available
SELECTION AGENT (mutation assays): No data available
SPINDLE INHIBITOR (cytogenetic assays): No data available
STAIN (for cytogenetic assays): No data available
NUMBER OF REPLICATIONS: No replications were performed
NUMBER OF CELLS EVALUATED: No data available
DETERMINATION OF CYTOTOXICITY
- Method: mitotic index; cloning efficiency; relative total growth; other: No data available
OTHER EXAMINATIONS:
- Determination of polyploidy: No data available
- Determination of endoreplication: No data available
- Other: No data available
OTHER: No data available - Evaluation criteria:
- Increase in the number of revertants, Toxicity toward the Ames Salmonella TA100 strain used was roughly estimated by visual comparison of the background lawn on the plate under a microscope, with 100% representing the same number of bacteria per unit area as the blank with no test solution (no toxicity) and 0 representing death of all bacteria.
- Statistics:
- No data available
Results and discussion
Test results
- Species / strain:
- S. typhimurium TA 100
- Metabolic activation:
- not specified
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- not specified
- Untreated negative controls validity:
- not specified
- Positive controls validity:
- not specified
- Remarks on result:
- other: strain/cell type:
- Remarks:
- Migrated from field 'Test system'.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative
The test compound failed to induce mutation in the Salmonella typhimurium TA100 and hence is not a mutagen. - Executive summary:
Ames assay was performed to evaluate the mutagenic nature of the test compound m-Methoxyacetophenone usingSalmonella typhimuriumTA100. The test compound was tested at dose levels of 103, 102, 101, 100and 10-1ug per plate.
The test compoundm-Methoxyacetophenonefailed to induce mutation in theSalmonella typhimuriumTA100 and hence is not a mutagen.
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