Hydrogen [1-[(2-hydroxy-4-nitrophenyl)azo]-2-naphtholato(2-)][1-[(2-hydroxy-5-nitrophenyl)azo]-2-naphtholato(2-)]chromate(1-) , compound with 3-[(2-ethylhexyl)oxy]propylamine (1:1)

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1994

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: well performed OECD study with GLP

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1994

Report date:

1994

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Hydrogen [1-[(2-hydroxy-4-nitrophenyl)azo]-2-naphtholato(2-)][1-[(2-hydroxy-5-nitrophenyl)azo]-2-naphtholato(2-)]chromate(1-) , compound with 3-[(2-ethylhexyl)oxy]propylamine (1:1)

EC Number:

276-857-1

EC Name:

Hydrogen [1-[(2-hydroxy-4-nitrophenyl)azo]-2-naphtholato(2-)][1-[(2-hydroxy-5-nitrophenyl)azo]-2-naphtholato(2-)]chromate(1-) , compound with 3-[(2-ethylhexyl)oxy]propylamine (1:1)

Cas Number:

72812-34-1

Molecular formula:

C32H18CrN6O8.C11H25NO.H

IUPAC Name:

Chromate(1-), [1-[2-[2-(hydroxy-kO)-4-nitrophenyl]diazenyl-kN1]-2-naphthalenolato(2-)-kO][1-[2-[2-(hydroxy-kO)-5-nitrophenyl]diazenyl-kN1]-2-naphthalenolato(2-)-kO]-, hydrogen, compd. with 3-[(2-ethylhexyl)oxy]-1-propanamine (1:1:1)

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna (Crustacea, Cladocea) (Straus, 1820)
- Age at study initiation : younger than 24 hours


ACCLIMATION

- Type of food: Daily with a suspension of fresh water algae

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Test conditions

Test temperature:

18 - 22 °C

pH:

8.1 - 8.2

Dissolved oxygen:

9.0 - 9.6 mg O2/L

Nominal and measured concentrations:

A suspension with 100 mg/L was prepared and was filtered through a rough paper filter. In the filtrat 15.8 mg/L of the substance were meaured
100 mg/L nominal, 15.8 mg/L measured.
Dilution of the fitrat 1 : 1: 7.4 mg/L measured

Details on test conditions:

TEST SYSTEM
- Test vessel: 100 ml, all glass
- No. of organisms per vessel: 10 per vessel containing 80 ml medium
- No. of vessels per concentration : 2
- No. of vessels per control (replicates): 1
- No. of vessels per vehicle control (replicates): 1
- No feeding
- No aeration of the test solution


OTHER TEST CONDITIONS
- Photoperiod: 16 hous photoperiod daily

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Results with reference substance (positive control):

Acute immobilization of daphnia after 24 h an 48 h was performed with potassium dichromate in concentrations of 0, 0.1, 0.18, 0.32, 0.56, 1.0 and
1.8 mg/L. An 48h EC50 value of 0.47 mg/L was determined

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The substance does not induce acute immobilization to daphnia with the saturated solution. ( filtrate of 100 mg/L test item (15.8 mg/L measured))

Executive summary:

The substance was tested for acute immobilization to Daphnia magna according to OECD guideline 202.

In the test the daphnia were exposed for 48 hours to 50 and 100 % (v/v) of a rough filtrate prepared from a suspension with 100 mg/L of the test item.. The test was performed in duplicate with 10 daphnia per vessel.

The substance did not induce acute immobilization of Daphnia magna at a supersaturated solution of the test item (100 mg/L nominal, 15.8 mg/L measured).