Hydrogen [1-[(2-hydroxy-4-nitrophenyl)azo]-2-naphtholato(2-)][1-[(2-hydroxy-5-nitrophenyl)azo]-2-naphtholato(2-)]chromate(1-) , compound with 3-[(2-ethylhexyl)oxy]propylamine (1:1)

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1994

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: well performed OECD study with GLP

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1994

Report date:

1994

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Hydrogen [1-[(2-hydroxy-4-nitrophenyl)azo]-2-naphtholato(2-)][1-[(2-hydroxy-5-nitrophenyl)azo]-2-naphtholato(2-)]chromate(1-) , compound with 3-[(2-ethylhexyl)oxy]propylamine (1:1)

EC Number:

276-857-1

EC Name:

Hydrogen [1-[(2-hydroxy-4-nitrophenyl)azo]-2-naphtholato(2-)][1-[(2-hydroxy-5-nitrophenyl)azo]-2-naphtholato(2-)]chromate(1-) , compound with 3-[(2-ethylhexyl)oxy]propylamine (1:1)

Cas Number:

72812-34-1

Molecular formula:

C32H18CrN6O8.C11H25NO.H

IUPAC Name:

Chromate(1-), [1-[2-[2-(hydroxy-kO)-4-nitrophenyl]diazenyl-kN1]-2-naphthalenolato(2-)-kO][1-[2-[2-(hydroxy-kO)-5-nitrophenyl]diazenyl-kN1]-2-naphthalenolato(2-)-kO]-, hydrogen, compd. with 3-[(2-ethylhexyl)oxy]-1-propanamine (1:1:1)

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Cyprinus carpio

Details on test organisms:

TEST ORGANISM
- Common name: Carp (Cyprinus carpio, Teleostei, Cyprinidae)
- Source: Zodiac, proefacc, "De Haar Vissen", L.U., Wageningen, the Netherlands
- Length at study initiation : 3.7 +- 0.1 cm
- Weight at study initiation : 1.83 +- 0.23 g
- no feeding 24 hours prior to the test and during the total test period


ACCLIMATION
- Acclimation period: at least 12 days after delivery
- Type of food: Trouvit or Artemia
- Feeding frequency: Daily
- Health during acclimation : less than 5% mortality seven days prior to the test start

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Post exposure observation period:

no

Test conditions

pH:

8.0 - 8.2

Dissolved oxygen:

8.9 - 9.3 mg O2/L

Nominal and measured concentrations:

100 mg/L nominal; 25 - 36 mg/ measured

Details on test conditions:

TEST SYSTEM
- Test vessel: 20 liter all glass vessel
- Aeration: continuously
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 2


TEST MEDIUM / WATER PARAMETERS
ISO-medium, aerated until the dissolved oxygen concentratin had reached saturation and the pH had stabilized. After aeration the hardness was 250 mg VaCO3 per liter and the pH was 8.2

OTHER TEST CONDITIONS
- Photoperiod: 16 hours photoperiod daily

Reference substance (positive control):

yes

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 2

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

- Behavioural abnormalities:
- Observations on body length and weight:
- Other biological observations:
- Mortality of control:
- Other adverse effects control:
- Abnormal responses:
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values:
- Effect concentrations exceeding solubility of substance in test medium:

Results with reference substance (positive control):

Positive control was performed with pentachlorophenol. Dtermined LC is between 0.18 and 0.32 mg/L

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The LC50 for fish is greater than 100 mg/L (nominal)

Executive summary:

The substance was tested for toxicity to fish according to OECD guuideline 203. A range finding test with 3 fish per concentration showed no mortality up to a concentration of 100 mg/L nominal. For the final test 7 carps per concentration were used at a concentration of 100 mg/L. No mortality was seen.

Under the conditions of the test the 96h LC50 for carp exposed to the test item was greater than the maximum solubility of the substance in water. Further, carp were not effected by exposure to a supersaturated solution of nominally 100 mg/L, corresponding with exposure of 25 - 36 mg/L.