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Environmental fate & pathways

Bioaccumulation: aquatic / sediment

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Administrative data

Endpoint:
bioaccumulation in aquatic species: fish
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
data from handbook or collection of data
Justification for type of information:
Data is from J-CHECK and Envichem authoritative database

Data source

Referenceopen allclose all

Reference Type:
other: authoritative database
Title:
Bioaccumulation: aquatic/sediment
Author:
National Institute of Technology and Evaluation.
Year:
2017
Bibliographic source:
Japan chemicals collaborative knowledge database (J-check), 2017
Reference Type:
other: authoritative database
Title:
Bioaccumulation: aquatic/sediment
Author:
Data bank of Environmental Properties of Chemicals (EnviChem)
Year:
2014
Bibliographic source:
Data bank of Environmental Properties of Chemicals (EnviChem), 2014.

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
other: "Bioaccumulation test of a chemical substance in fish or shellfish" provided in "the Notice on the Test Method Concerning New Chemical Substances"
Principles of method if other than guideline:
Bioaccumulation study was conducted on test organism Cyprinus carpio for 8 weeks for evaluating the bioconcentration factor (BCF value) of substance N, N-diethylaniline. The study was performed according to other guideline "Bioaccumulation test of a chemical substance in fish or shellfish" provided in "the Notice on the Test Method Concerning New Chemical Substances".
GLP compliance:
not specified

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
liquid
Specific details on test material used for the study:
- Name of test material: N, N-diethylaniline
- Molecular formula: C10H15N
- Molecular weight: 149.236 g/mol
- Smiles notation: c1(N(CC)CC)ccccc1
- InChl: 1S/C10H15N/c1-3-11(4-2)10-8-6-5-7-9-10/h5-9H,3-4H2,1-2H3
- Substance type: Organic
- Physical state: Liquid
- Water solubility: slightly soluble
Radiolabelling:
not specified

Test solutions

Vehicle:
yes
Details on preparation of test solutions, spiked fish food or sediment:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
Chemical name of vehicle: HCO-40, 1st Concentration area: 1.00 ppm, 2nd Concentration area: 0.1 ppm

Test organisms

Test organisms (species):
Cyprinus carpio

Study design

Route of exposure:
aqueous
Test type:
static
Water / sediment media type:
natural water: freshwater
Total exposure / uptake duration:
8 wk

Test conditions

Details on test conditions:
RANGE-FINDING / PRELIMINARY STUDY
- Test concentrations: TLm (48 hr) 16.8 ppm (16.8 mg/l) (w/v), Rice fish (Oryzias latipes)
Nominal and measured concentrations:
Nominal concentrations:
1st concentration area: 0.2 ppm (0.2 mg/l) (w/v)
2nd concentration area: 0.02 ppm (0.02 mg/l) (w/v)
Reference substance (positive control):
not specified

Results and discussion

Bioaccumulation factoropen allclose all
Conc. / dose:
0.2 mg/L
Type:
BCF
Value:
44 L/kg
Basis:
whole body w.w.
Time of plateau:
8 wk
Remarks on result:
other: Other details not known
Conc. / dose:
0.2 mg/L
Type:
BCF
Value:
161 L/kg
Basis:
whole body w.w.
Time of plateau:
8 wk
Remarks on result:
other: Other details not known
Conc. / dose:
0.02 mg/L
Type:
BCF
Value:
17 L/kg
Basis:
whole body w.w.
Time of plateau:
8 wk
Remarks on result:
other: Other details not known
Conc. / dose:
0.02 mg/L
Type:
BCF
Value:
125 L/kg
Basis:
whole body w.w.
Time of plateau:
8 wk
Remarks on result:
other: Other details not known

Any other information on results incl. tables

Bioaccumulation test results:

 

 

2w

3w

4w

6w

1st Concentration area

 

Test substance concentration (ppm)

0.166

0.157

0.161

0.162

Bioconcentration factor (L/kg)  

75

95

44

161

63

118

57

64

2nd Concentration area

 

Test substance concentration (ppm)

0.021

0.020

0.019

0.020

Bioconcentration factor (L/kg)  

98

57

28

125

80

17

29

75

Applicant's summary and conclusion

Validity criteria fulfilled:
not specified
Conclusions:
The bioconcentration factor (BCF value) of substance N, N-diethylaniline on Cyprinus carpio was determined to be 44 and 161 L/Kg at a conc. of 0.2 mg/l and 17 and 125 L/Kg at a conc. of 0.02 mg/l, respectively.
Executive summary:

Bioaccumulation study was conducted on test organism Cyprinus carpio for 8 weeks for evaluating the bioconcentration factor (BCF value) of substance N, N-diethylaniline (CAS no. 91-66-7) under static conditions.The study was performed according to other guideline"Bioaccumulation test of a chemical substance in fish or shellfish" provided in "the Notice on the Test Method Concerning New Chemical Substances". Cyprinus carpio was used as a test organism for the study. HCO-40 was used as a vehicle. Test chemical nominal conc. used for the study were 0.2 mg/l and 0.02 mg/l (w/v), respectively. Range finding study involve the TLm (48 hr) of 16.8 ppm (16.8 mg/l) (w/v) on Rice fish (Oryzias latipes). The bioconcentration factor (BCF value) of substance N, N-diethylaniline on Cyprinus carpio was determined to be 44 and 161 L/Kg at a conc. of 0.2 mg/l and 17 and 125 L/Kg at a conc. of 0.02 mg/l, respectively, which does not exceed the bioconcentration threshold of 2000, indicating that the chemical N, N-diethylaniline is not expected to bioaccumulate in the food chain.