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EC number: 415-490-5 | CAS number: 141773-73-1 HELVETOLIDE
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Hydrolysis
Administrative data
Link to relevant study record(s)
- Endpoint:
- hydrolysis
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From 20 February 1995 to 15 July 1995
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
- Remarks:
- The study is deemed scientifically valid even though some information were not reported, such as the repeatability and sensitivity of analytical method and the characteristics of water used. Also no positive or negative control was used and the no sterility check was reported.
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 111 (Hydrolysis as a Function of pH)
- Deviations:
- no
- Principles of method if other than guideline:
- Not applicable
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- - Storage condition of test material: room temperature
- Radiolabelling:
- no
- Analytical monitoring:
- yes
- Details on sampling:
- - Sampling method: an aliquot of each sample solution (100 mL) was extracted using three portions (3 x 25 mL) of dichloromethane. The extracts were passed through anhydrous sodium sulphate, combined and evaporated to dryness. The residue was dissolved in hexane (4.0 mL).
- Sampling intervals for the parent/transformation products:
# Preliminary Test (Test1): 0, 2.4, 48, 120 and 288 hours
# Test 3: 0, 24, 166, 243, 337 and 411 hours - Buffers:
- pH 4:
- Composition of buffer:
Citric acid: 0.05 mol/L
Sodium hydroxide: 0.10 mol/L
Hydrochloric acid: 0.05 mol/L
pH 7:
- Composition of buffer:
Disodium hydrogen orthophosphate: 0.04 mol/L
Potassium dihydrogen orthophosphate: 0.03 mol/L
pH 9:
- Composition of buffer:
Disodium tetraborate: 0.05 mol/L
Hydrochloric acid: 0.0192 mol/L - Estimation method (if used):
- None
- Details on test conditions:
- TEST SYSTEM
- Type, material and volume of test flasks, other equipment used: glass flasks
- Measures taken to avoid photolytic effects: protected from light
- Measures to exclude oxygen: stoppered flasks
- If no traps were used, is the test system closed/open: closed system (stoppered flasks)
- Is there any indication of the test material adsorbing to the walls of the test apparatus? no
TEST MEDIUM
- Volume used/treatment: 1000 mL
- Kind and purity of water: not mentioned
- Preparation of test medium: A stock solution of the test material was prepared in acetonitrile at a concentration of 150 mg/L. Aliquots (10.0 mL) of the stock solutions were added to each buffer solutions (1000 mL) to give a nominal concentration of 1.5 mg/L
- Renewal of test solution: no - Duration:
- 288 h
- pH:
- 4
- Temp.:
- 50 °C
- Initial conc. measured:
- ca. 1.52 mg/L
- Duration:
- 288 h
- pH:
- 7
- Temp.:
- 50 °C
- Initial conc. measured:
- ca. 1.59 mg/L
- Duration:
- 120 h
- pH:
- 9
- Temp.:
- 50 °C
- Initial conc. measured:
- ca. 1.86 mg/L
- Duration:
- 411 h
- pH:
- 9
- Temp.:
- 25 °C
- Initial conc. measured:
- ca. 1.61 mg/L
- Number of replicates:
- 1
- Positive controls:
- no
- Negative controls:
- no
- Statistical methods:
- Log10 regression
- Preliminary study:
- No hydrolysis was measured in pH4 and pH7 buffer solutions at 50°C. Greater than 10% hydrolysis was observed after 5 days at 50°C in pH9 buffer solution.
- Test performance:
- None
- Transformation products:
- not measured
- Details on hydrolysis and appearance of transformation product(s):
- Not applicable
- % Recovery:
- ca. 93.8
- pH:
- 9
- Temp.:
- 25 °C
- Duration:
- ca. 24 h
- % Recovery:
- ca. 72.6
- pH:
- 9
- Temp.:
- 25 °C
- Duration:
- ca. 166 h
- % Recovery:
- ca. 45
- pH:
- 9
- Temp.:
- 25 °C
- Duration:
- ca. 243 h
- % Recovery:
- ca. 37.2
- pH:
- 9
- Temp.:
- 25 °C
- Duration:
- ca. 337 h
- % Recovery:
- ca. 30
- pH:
- 9
- Temp.:
- 25 °C
- Duration:
- ca. 411 h
- Key result
- pH:
- 4
- Temp.:
- 25 °C
- DT50:
- > 1 yr
- Type:
- not specified
- Key result
- pH:
- 7
- Temp.:
- 25 °C
- DT50:
- > 1 yr
- Type:
- not specified
- Key result
- pH:
- 9
- Temp.:
- 25 °C
- Hydrolysis rate constant:
- ca. 8.6 s-1
- DT50:
- ca. 224 h
- Type:
- (pseudo-)first order (= half-life)
- Remarks on result:
- other: r2=0.987
- Other kinetic parameters:
- None
- Details on results:
- TEST CONDITIONS
- pH, sterility, temperature, and other experimental conditions maintained throughout the study: not reported - Validity criteria fulfilled:
- yes
- Conclusions:
- The rate constant of hydrolysis of the test substance at pH9 and at 25°C has been determined to be 8.60 x 10-7 s-1 (half-life = 224 hours). At pH 4 and 7 the half-life is estimated to be superior to 1 year.
- Executive summary:
A study was performed to assess the abiotic degradation of the test substance, according to a method equivalent to OECD Guideline 111 (Hydrolysis as a Function of pH). No major deviation was observed even though some information are lacking such as the repeatability and sensitivity of analytical method.
The transformation products were not identified.
Hydrolysis was evaluated in a preliminary test and then in a main test. In the preliminary test the substance failed to hydrolyse at pH 4 and pH 7 (at 50°C) while more than 10% hydrolysis was observed after 5 days in pH 9 (at 50°C).
Therefore the main test was performed at pH 9 and directly at 25°C.
The Log10regressions suggest that the reactions are pseudo-first order because Log10 concentration versus time is linear (r2> 0.98).
The rate constant at pH 9 and 25°C was measured at 8.60 x 10-7s-1, which corresponds to a half-life of 224 hours.
The rate constant at pH 9 and 50°C was measured at 3.58x 10-2h-1, which corresponds to a half-life of 19.4 hours.
Reference
Table 5.1.2/1: Preliminary test (test 1) results
Time (hours) | pH4 | pH7 | pH9 | |||
Concentration (mg/L) | % recovery (expressed as % of initial) | Concentration (mg/L) | % recovery (expressed as % of initial) | Concentration (mg/L) | % recovery (expressed as % of initial) | |
Initial | 1.52 | - | 1.59 | - | 1.86 | - |
2.4 | 1.35 | 88.8 | 1.52 | 96.0 | 1.32 | 70.6 |
24 | 1.31 | 86.4 | 1.24 | 18.4 | 0.634 | 34.1 |
48 | 1.1 | 72.3 | 1.25 | 79.1 | 0.292 | 15.7 |
120 | 1.22 | 80.2 | 1.28 | 81.0 | 0.0221 | 1.19 |
288 | 1.46 | 96.0 | 1.29 | 81.5 |
The log10 regressions at pH 9 50°C and pH 9 25°C are presented in attached figure.
Description of key information
Method equivalent to OECD Guideline 111, GLP, key study, validity 2:
Half-life = 224 hours (pH 9; 25°C)
Key value for chemical safety assessment
- Half-life for hydrolysis:
- 224 h
- at the temperature of:
- 25 °C
Additional information
One valid key study is available to assess the abiotic degradation of the registered substance. This study was performed according to a method equivalent to OECD Guideline 111 with GLP statement. No major deviation was observed even though some information are lacking such as the repeatability and sensitivity of analytical method.
Hydrolysis was evaluated in a preliminary test and then in a main test. In the preliminary test the substance failed to hydrolyse at pH 4 and pH 7 (at 50°C) while more than 10% hydrolysis was observed after 5 days in pH 9 (at 50°C). Therefore the main test was performed at pH 9 and directly at 25°C. The Log10regressions suggest that the reactions are pseudo-first order because Log10 concentration versus time is linear (r2> 0.98). The rate constant at pH 9 and 25°C was measured at 8.60 x 10-7s-1, which corresponds to a half-life of 224 hours. The rate constant at pH 9 and 50°C was measured at 3.58x 10-2h-1, which corresponds to a half-life of 19.4 hours. The transformation products were not identified.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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