6,18-dihydrodinaphtho[2,3-i:2',3'-i']benzo[1,2-a:4,5-a']dicarbazole-5,7,12,17,19,24-hexone

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

02 August, 1993 to 10 November, 1993

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Deviations:

no

GLP compliance:

yes

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Identification: FAT 46014 / G
- Lot/batch: 258645.16
- Purity test date: 99.8 % AS
- Apperance : Red solid

Analytical monitoring:

yes

Details on sampling:

Composite samples of each test concentration were drawn by mixing identical volumes of the test solutions taken from the approximate center of the test vessels. They were taken immediately before exposure and after 48 hours exposure and kept at - 18 °C to - 22 °C until analysis.

Vehicle:

no

Details on test solutions:

Stock solution: 0.99 g test substance were mixed with 9.02 g water, containing 0.4 % ARKOPAL by means of a marble mill during 24 hours. 1.00 g of this blend were mixed and made up to 1000 ml with water.

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Strain:Straus 1820
- Source:CIBA-GEIGY Ltd., testing facility
- Method of breeding:Cultures of daphnia are maintained in glass vessels containing approx. 2.5 L of reconstituted water (see below) at 20 +/- 1 °C (water is renewed partially thrice weekly). At each renewal the daphnia are fed with a suspension of green algae (Scenedesmus subspicatus) supplemented by a suspension of TETRAMIN-extract in such quantities that the food is consumed after 24 h.
- Feeding during test : None during the test

ACCLIMATION
- Acclimation period: 24 hours before the begin of the exposure reproductive daphnia are separated from the young by sieving all individuals through a 800 µm sieve. This operation is repeated 6 hours before the exposure and the young (6-24 h of age) are retained for the test.

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Post exposure observation period:

None

Hardness:

240 mg CaC03/l.

Test temperature:

20 +/ - 1 °C

pH:

None

Dissolved oxygen:

None

Salinity:

None

Nominal and measured concentrations:

Test concentrations:
Nominal: 4.3, 9.4, 20.7, 45.5 and 100 mg test substance/l
Initial: 3.5, 4.5, 7.7, 13.5 and 17.1 mg test substance/l
Controls:
Blank: water

Details on test conditions:

TEST SYSTEM
- Test vessel:Beakers covered with watch glasses 100 ml solution per beaker
- Aeration:The water was aerated with clean air for at least 24 hours before use.

TEST MEDIUM / WATER PARAMETERS
Reconstituted water containing
65 mg NaHC03
294 mg CaCl2 • 2 H20
123 mg MgS04 • 7 H20
6 mg KCl
in 1000 ml bidistilled water.

OTHER TEST CONDITIONS
- Light intensity: Fluorescent light, 16 hours daily approx. 1500 lux

Vehicle: 3.6 mg alkylphenol-polyglycolether (ARKOPAL)/l

Reference substance (positive control):

not specified

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 17.1 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

> 17.1 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

17.1 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

17.1 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

> 17.1 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

The test substance settled down during equilibration (7 hours), exposure and an uptake of test substance by the daphnids was observed (coloured animals) without effecting the daphnids therefore values are based on initial concentrations.

Results with reference substance (positive control):

None

Reported statistics and error estimates:

None

None

Validity criteria fulfilled:

yes

Conclusions:

The EC50 value was determined to be >17.1 mg/L at 48 h of exposure.

Executive summary:

A study was performed to check the acute toxicity in aquatic invertebrates. The study was performed for the test item using species Daphnia magna. The method followed was C.2 of Commission Regulation (EC) No. 440/2008 which is compatible with OECD Guidelines for Testing of Chemicals No 202. The test was performed in compliance with Good Laboratory Practice Regulations.

The exposure time of FAT 46014 / G to daphnia magna was 24 and 48 hours. The nominal test concentrations used were 4.3, 9.4, 20.7, 45.5 and 100 mg test substance/L, which were found to correspond to initial measured concentrations of 3.5, 4.5, 7.7, 13.5 and 17.1 mg test substance/L. No immobilisation was observed at any of the exposure concentrations. Hence, the EC50 value was determined to be >17.1 mg/L at 48 h of exposure. The No Observed Effect Concentration was 17.1 mg/L at 48 h of exposure.

Description of key information

EC50 (48 h) of FAT 46014/G was found to be greater than 17.1 mg/l

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

17.1 mg/L

Additional information

A study was performed to check the acute toxicity in aquatic invertebrates. The study was performed for the test item using species Daphnia magna. The method followed was C.2 of Commission Regulation (EC) No. 440/2008 which is compatible with OECD Guidelines for Testing of Chemicals No 202. The test was performed in compliance with Good Laboratory Practice Regulations.

The exposure time of FAT 46014/G to daphnia magna was 48 hours. The nominal test concentrations used were 4.3, 9.4, 20.7, 45.5 and 100 mg test substance/L, which were found to correspond to initial measured concentrations of 3.5, 4.5, 7.7, 13.5 and 17.1 mg test substance/L. No immobilisation was observed at any of the exposure concentrations. Hence, the EC50 value was determined to be >17.1 mg/L at 48h of exposure. The No Observed Effect Concentration was 17.1 mg/L at 48 h of exposure.