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EC number: 219-371-7 | CAS number: 2425-79-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
Description of key information
The substance was not acutely toxic to fish when tested according to OECD 203. The calculated 96-h EC50 is 19.8 mg/L; calculated from the geometric average concentrations of the EC0 (14.3 mg/L) and the EC100 (25.4 mg/L) assuming 37% loss over the course of the 96-hour fish assay at 22 °C.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 19.8 mg/L
Additional information
Acute toxicity to Brachydanio rerio (new name: Danio rerio) was assessed according to OECD 203 by a study not following GLP procedures (Rufli, 1989). Fish were exposed under static conditions to the substance at a range of concentrations from 18, 32, 58, and 100 mg/L in freshwater. The test was performed with 10 fish per vessel and concentration. For each concentration, the percentage of mortality at 24, 48, 72, and 96 hours was recorded. The following OECD 203 validity criteria were met: mortality in the control did not exceed 10% at the end of the test; constant conditions (temperature and pH) were within specified deviations; and the dissolved oxygen concentration was at least 60% of the air saturation value throughout the test. The OECD 203 validity criterion, concerning evidence that the concentration of the substance was at least 80% of the nominal concentration throughout the test, was not met.
Štreit (2010) determined the water solubility to be 55.6 g/L, which is three orders of magnitude higher than the nominal 96-h LC50 in fish, clearly demonstrating that the test substance was soluble at the concentrations used in the study. However, evidence of hydrolysis was reported in the water solubility report, and a review of “Fig. No. 1:LC/MS analysis of Grilonit 1/10 water solution” in the Annexes revealed that the primary peak (Retention Time = 10.2 mins) fell from ~3.5 to ~2.2 over the course of the 4-day experiment (3 days at 30 °C + 1 at 20 °C). This amounts to 37% loss of the primary component over this 96-hour period, which is most probably due to ring-opening hydrolysis of the epoxy groups.
Assuming the same 37% loss over the course of the fish study (96-h at 22 °C) and assuming that the actual concentrations were as follows:
Initial (= nominal) [mg/L] |
10 |
18 |
32 |
58 |
100 |
Final (96-h) [mg/L] |
6.3 |
11.3 |
20.1 |
36.5 |
62.9 |
Geometric average [mg/L] |
7.9 |
14.3 |
25.4 |
46.0 |
79.3 |
The 96-h effect concentrations would be:
Duration | Endpoint |
Effect conc. (mg/L) |
Nominal/Measured |
Conc. based on |
Basis for effect |
Remarks |
96-h |
LC50 |
19.8 |
Meas. (geom.. mean)
(Nominal value was 24 mg/L) |
test mat. |
mortality |
Calculated from the geometric average concentrations, (half-way between 14.3 and 25.4 mg/L). |
96-h |
LC0 |
14.3 |
Meas. (geom.. mean)
(Nominal value was 18 mg/L) |
test mat. |
mortality |
Assuming 37% loss over the course of the fish study (96-h at 22 °C) |
96-h |
LC100 |
25.4 |
Meas. (geom.. mean)
(Nominal value was 32 mg/L) |
test mat. |
mortality |
Assuming 37% loss over the course of the fish study (96-h at 22 °C) |
This estimate is conservative in that it assumes as much hydrolysis at 4-days at 22 °C as was seen at 3-days at 30 °C + 1-day at 20 °C.
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