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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2002-03-04 to 2003-05-21
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: OECD Guideline study under GLP

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2003
Report date:
2003

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
no
GLP compliance:
yes (incl. QA statement)

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): 1,1,3-Trichloraceton
- Substance type: Industrial chemical
- Physical state: liquid
- Purity test date: not given in report
- Lot/batch No.: 1TC112013
- Expiration date of the lot/batch: 2002-12-31
- Stability under test conditions: stable
- Storage condition of test material: room temperature in the dark
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material (migrated information):
OTHER PROPERTIES (if relevant for this endpoint)
- Results of test for ready biodegradability: not readily biodegradable

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
- Concentrations: all
- Sampling method: One aliquod from the freshly prepared test meda of all concentrations was taken at the start of the test before filling the test vessels. One combined aliquod was collected from the test media of all 4 exposure vessels of all test concentrations at the end of the study to determine the stability.
-Storage of the samples: at -20 °C

Test solutions

Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTIONS
- Evidence of undissolved material (e.g. precipitate, surface film, etc): no

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: Daphnia magna
- Source: LPT Löhndorf, Germany
- Age at study initiation (mean and range, SD):
- Weight at study initiation (mean and range, SD):
- Length at study initiation (length definition, mean, range and SD):
- Valve height at study initiation, for shell deposition study (mean and range, SD):
- Peripheral shell growth removed prior to test initiation:
- Method of breeding:
- Feeding during test
- Food type:
- Amount:
- Frequency:


ACCLIMATION
- Acclimation period:
- Acclimation conditions (same as test or not):
- Type and amount of food:
- Feeding frequency:
- Health during acclimation (any mortality observed):


QUARANTINE (wild caught)
- Duration:
- Health/mortality:

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Remarks on exposure duration:
h
Post exposure observation period:
not stated in report

Test conditions

Hardness:
250 mg CaCO3
Test temperature:
20 +/- 1 °C
pH:
7.9 +/- 0.3
Dissolved oxygen:
> 80% of maximum saturation
Salinity:
freshwater
Nominal and measured concentrations:
0.01, 0.0215, 0.0464, 0.1, 0.215, 10 and 100 mg/l (nominal = measured)
Details on test conditions:
TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: 190 x 90 mm, 2 l
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 1


TEST MEDIUM / WATER PARAMETERS
- Ca/mg ratio: 4:1
- Culture medium different from test medium: no


OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 hours light 8 hours dark
- Light intensity: 500 lux +/- 20 %


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : EC50

Reference substance (positive control):
no

Results and discussion

Effect concentrationsopen allclose all
Duration:
24 h
Dose descriptor:
NOEC
Effect conc.:
0.01 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: not applicable
Duration:
24 h
Dose descriptor:
EC0
Effect conc.:
0.215 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: not applicable
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
> 0.215 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: not applicable
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
0.01 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: not applicable
Duration:
48 h
Dose descriptor:
EC0
Effect conc.:
0.021 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: not applicable
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
0.12 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: 0.08 - 0.24 mg/l
Details on results:
- Behavioural abnormalities: not observed
- Observations on body length and weight: not give in report
- Mortality of control: 0
- Other adverse effects control: none
- Abnormal responses: none
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: no
- Effect concentrations exceeding solubility of substance in test medium: no
Results with reference substance (positive control):
not available
Reported statistics and error estimates:
The EC50 (48 h) was calculated by linear regression analysis employing probit analysis. The EC50 (24 h), NOEC values were taken directly from the raw data.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
1,1,3 -Trichloroacetone is very toxic for the aquatic invertebrate Daphnia magna under the con-ditions used in this test
Executive summary:

Young Daphnia were exposed in a static immobilisiation test according to OECD guideline 202 in order to evaluate the influence of 1,1,3 -Trichloroacetone on the mobility and survival. Daphnia magna were exposed in a static test for 48 hours to the test medium which contained the test substance up to 100 mg/l in test water.

The test medium of the highest concentration of 100 mg/l exposure medium was prepared by mixing 100 mg test item into 1 l test water by intense stirring for approx. 30 minutes. The solution obtained was user as stock solution for lower concentrated test media.

The nominal concentrations tested were 0.01, 0.0215, 0.0464, 0.100, 0.215 10 and 100 mg/l. Aditionally a control was tested in parallel. Aliquots were taken at the beginning and end of the exposure period for GC-analysis. The GC-analysis revealed correctly prepared exposure media. The analytical results obtained were within +/- 20 % of the nominal concentrations. The reported biological results are related to nominal concentrations.

The NOEC was 0.0100mg/l (24 h and 48 h), the EC50 was 0.215 mg/l (24 h) and 0.12 mg/l (48 h with a 95% confidence interval of 0.08-0.024 mg/l). It could be stated that 1,1,3 -Trichloroacetone is very toxic for the aquatic invertebrate Daphnia magna under the conditions used in this test.