Registration Dossier

Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
supporting study
Study period:
1989
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Comparable to OECD Test Guidance

Data source

Reference
Reference Type:
publication
Title:
Unnamed
Year:
1990
Report date:
1998

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Deviations:
no
GLP compliance:
not specified

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): 1,1,3-Trichloroacetone
- Physical state: liquid
- Analytical purity: 97%
- Impurities (identity and concentrations): not reported
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material (migrated information):
not applicable

Sampling and analysis

Analytical monitoring:
no
Details on sampling:
not applicable

Test solutions

Vehicle:
no
Details on test solutions:
not reported

Test organisms

Test organisms (species):
Oncorhynchus mykiss (previous name: Salmo gairdneri)
Details on test organisms:
TEST ORGANISM
- Common name: rainbow trout
- Source: laboratory reared population
- Age at study initiation (mean and range, SD): juvenile


ACCLIMATION
- Acclimation period:appropriate
- Acclimation conditions (same as test or not): same

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Post exposure observation period:
not reported

Test conditions

Hardness:
4.5 mg CaCO3/l
Test temperature:
15 °C
pH:
6.4
Dissolved oxygen:
not reported
Salinity:
not reported
Nominal and measured concentrations:
up to 25 mg/l
Details on test conditions:
TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: glas
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2
- Biomass loading rate: 0.55 g/l


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Vancouver tap water
- Chlorine: < 0.01 mg/l
- Alkalinity: 3.8 mg CaCO3/l
- Conductivity: 15 µmhos/cm
- Culture medium different from test medium: no


OTHER TEST CONDITIONS
- Photoperiod: 12/12
Reference substance (positive control):
yes
Remarks:
sodium pentachlorophenate

Results and discussion

Effect concentrations
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
2.3
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Remarks on result:
other: 95% CL 2 to 3 mg/l
Details on results:
not observed
Results with reference substance (positive control):
- Results with reference substance valid? yes
- Mortality: yes
-LC50 96.7 to 104.9 µg/l
Reported statistics and error estimates:
no

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
1,1,3-Trichloroacetone is toxic for fish under the conditions used.
Executive summary:

1,1,3-Trichloroacetone was assessed for its toxic properties in a static acute fish toxicity test. Groups of 10 fish were exposed to the test item up to concentrations of 25 mg/l of Vancouver tap water. Two replicates were used for each concentration. The positive control (sodium pentachlorophenate) showed an LC50 of 96.7 to 104.9 µg/l. The LC50 for the test item is 2.3 mg/l. 1,1,3-Trichloroacetone is toxic for fish under the conditions used.