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EC number: 412-550-2 | CAS number: 85776-14-3 GRAPHTOL ORANGE 3RT; PERMANENT ORANGE 2 RLD; PERMANENT ORANGE 3 RTN
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Short-term toxicity to fish
Administrative data
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1993
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Study performed according to relevant guidelines and compliant to GLP
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 993
- Report date:
- 1993
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: OCDE n° 203 (1984) Directive CEE 84/449 (1984) - annexe V - méthode C1
- GLP compliance:
- yes
Test material
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
Test solutions
- Vehicle:
- no
Test organisms
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
Test conditions
- Hardness:
- 90 mg CaCO3/L
- Test temperature:
- 20.2°C - 22.6°C
- pH:
- 6.63 - 7.47
- Dissolved oxygen:
- at least 82% of the air saturation value
- Nominal and measured concentrations:
- limit concentration: 1000 mg/L (nominal)
- Details on test conditions:
- The test media were heterogeneous supensions of the test substance. These suspensions were prepered approx. 72 hours prior to the start of the exposure, submitted to magnetic stirring for 1h 45 min and allowed to settle until the beginning of the exposure. The test substance particles forming deposit at the tank bottom and onto glass tank surfaces, and floating at the surface of the experimental water. Coloration of the test media was due to test substance in suspension.
Periodic manual stirring and air bubbling allowed, as far as possible, dispersion of the test substance particles in the test media throughout the study period. - Reference substance (positive control):
- no
Results and discussion
Effect concentrations
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: theoretical concentration
- Details on results:
- No mortality was recorded during the 96-hour study periode, until the theoretical concentration of 1000 mg/L. The water solubility of the test item is < 0.1 mg/L. Therefore, no mortality of the saturated solution was recorded during the 96-hour study periode.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- LC50 - 96 hours for fish in freshwater medium: higer than 1000 mg/L
- Executive summary:
Freshwater fish (Brachydanio rerio) were exposed to the test substance for 96 hours.
A range-finding study (phase I) was performed using the concentrations of 10 - 50 - 100 - 500 and 1,000 mg of test substance per litre of test medium (10 Brachydanio rerio per group). A control group (not exposed to any substance) was also employed under the same experimental conditions.
No mortality was recorded during the 96-hour study period, until 1,000 mg of test substance per litre of test medium. The range-fmding study was then considered as a limit test, and there was no need to perform an LC 50 evaluation above this concentration.
Any death and behavioural abnormalities (problems of swimming, equilibrium, pigmentation, fin morphology, ... ) were recorded approximately 2 hours, then 24, 48, 72 and 96 hours after the start of the study.
Measurements of pH, dissolved oxygen and temperature were performed at the start of the study for each test medium and the control, then approximately 24,48,72 and 96 hours later.
The study was performed in a static system (without renewal of test media during the study period).
Each test medium has been twice sampled (at the start and at the end of the study).
The quality criteria for the study as notified in the O.E.C.D. and E.E.C. protocols relative to the dissolved
oxygen concentration and mortality in the control group were respected.
Concentration levels of the test substance throughout the study period was guaranteed by the physicochemical
stability of the test substance in the experimental conditions.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.

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