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Toxicological information

Genetic toxicity: in vitro

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Administrative data

Endpoint:
in vitro gene mutation study in bacteria
Remarks:
Type of genotoxicity: gene mutation
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Well documented publication which meets basic scientific principles

Data source

Reference
Reference Type:
publication
Title:
The results of microbial mutation test for forty-three industrial chemicals
Author:
Shimizu H, Suzuki Y, Takemura N, Goto S and Matsushita H
Year:
1985
Bibliographic source:
Jpn. J. Ind. Health., Vol 27

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
Ames test
GLP compliance:
no
Type of assay:
bacterial reverse mutation assay

Test material

Constituent 1
Chemical structure
Reference substance name:
Pentasodium triphosphate
EC Number:
231-838-7
EC Name:
Pentasodium triphosphate
Cas Number:
7758-29-4
Molecular formula:
H5O10P3.5Na
IUPAC Name:
pentasodium triphosphate
Details on test material:
- Name of test material (as cited in study report): Pentasodium triphosphate
- Purity: 90%

Method

Species / strain
Species / strain / cell type:
other: S. typhimurium (TA100, TA98, TA1535, TA1537 and TA1538), E.coli (WP2uvrA)
Metabolic activation:
with and without
Metabolic activation system:
S9 mix
Test concentrations with justification for top dose:
5, 10, 50, 100, 500, 1000 and 5000 µg/plate
Vehicle / solvent:
- Vehicle(s)/solvent(s) used: water
Controls
Untreated negative controls:
yes
Remarks:
water
Negative solvent / vehicle controls:
yes
Remarks:
DMSO and acetone
Positive controls:
yes
Remarks:
AF-2, ENNG, 9AC, 4NQO B(a)P, 2AA
Details on test system and experimental conditions:
METHOD OF APPLICATION: preincubation


DURATION
- Preincubation period: 20 minutes
- Exposure duration: 48 hours


Results and discussion

Test resultsopen allclose all
Species / strain:
S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
Metabolic activation:
with and without
Genotoxicity:
negative
Cytotoxicity / choice of top concentrations:
not specified
Vehicle controls validity:
not applicable
Species / strain:
S. typhimurium TA 1538
Metabolic activation:
with and without
Genotoxicity:
negative
Cytotoxicity / choice of top concentrations:
not specified
Species / strain:
E. coli WP2 uvr A
Metabolic activation:
with and without
Genotoxicity:
negative
Cytotoxicity / choice of top concentrations:
not specified
Vehicle controls validity:
not applicable
Remarks on result:
other: all strains/cell types tested
Remarks:
Migrated from field 'Test system'.

Any other information on results incl. tables

Table 1: Revertant colonies/plates (without S9 -mix)

Revertant colonies/plates (Mean ± S.D)

Without S9-mix

µg/plate

TA100

TA 1535

WP2 uvr A 

TA98

TA1537

TA1538

H20 (control)

149 ± 17.1

28 ± 6.9

32 ± 7.3

29 ± 6.2

16 ± 6.4

21 ± 5.5

DMSO (control)

150 ± 16.8

30 ± 5.6

30 ± 9.7

 32 ± 7.7

18 ± 8.6

22 ± 7.3

Acetone (control)

179 ± 15.0

23 ± 1.5

30 ± 7.5

24 ± 3.0

10 ± 3.0

21 ± 0.5

AF-2 (0.01)

501 ± 84.7

-

-

-

-

-

AF-2 (0.05)

-

-

1082 ± 293.7

278 ± 64.8

-

-

ENNG

-

1101 ± 683.1

-

-

-

-

9AC

-

-

-

-

889 ± 275.7

-

4NQO

-

-

-

-

-

270 ± 66.2

B(a)P

-

-

-

-

-

-

2AA

-

-

-

-

-

-

5

175

29

22

30

11

21

10

166

26

25

30

20

26

50

167

29

24

37

16

27

100

170

21

30

23

19

25

500

153

26

30

26

21

27

1000

158

24

32

30

13

24

5000

171

21

29

33

12

25

Table 2: Revertant colonies/plate (with S9 mix)

Revertant colonies/plates (Mean ± S.D)

With S9-mix

Dose

µg/plate

TA100

TA 1535

WP2 uvr A 

TA98

TA1537

TA1538

H20 (control)

161 ± 16.2

15 ± 3.6

33 ± 10.3

39 ± 8.6

21 ± 8.1

28 ± 7.0

DMSO (control)

154 ± 17.5

15 ± 7.1

34 ± 10.9

 42 ± 10.1

22 ± 6.3

28 ± 5.7

Acetone (control)

136 ± 7.0

8 ± 1.5

26 ± 4.0

32 ± 1.0

18 ± 2.5

26 ± 1.5

AF-2 (0.01)

-

-

-

-

-

-

AF-2 (0.05)

-

-

-

-

-

-

ENNG

-

-

-

-

-

-

9AC

-

-

-

-

-

-

4NQO

-

-

-

-

-

-

B(a)P

1084 ± 236.3

-

-

809 ± 108.4

313 ± 41.6

354 ± 89.4

2AA

-

440 ± 198.6

359 ± 127.0

-

-

-

5

170

16

33

54

23

24

10

172

15

29

63

33

22

50

158

18

27

53

31

19

100

165

19

37

48

25

26

500

182

14

27

49

26

28

1000

175

14

24

53

17

32

5000

166

17

23

59

18

21

- not tested

Positive controls:

2- (2-Furyl)-3-(5-nitro-2-furyl)acrylamide (AF-2), N-ethyl-N'-nitro-N-nitroguanidine (ENNG), 9-aminoacridine (9AC), 4-nitroquinoline-1-oxide (4NQO), benzo(a)pyrene (B(a)P), 2-aminoanthracene (2AA)

Applicant's summary and conclusion

Conclusions:
Interpretation of results (migrated information):
negative