Antimony nickel titanium rutile

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Remarks:

Rating in accordance with OECD SIDS (2002)

Data source

Reference

Reference 1

Reference Type:

study report

Title:

Unnamed

Year:

2001

Reference 2

Reference Type:

secondary source

Title:

SIDS - C.I. PIGMENT YELLOW 53

Author:

OECD

Year:

2002

Bibliographic source:

UNEP PUBLICATIONS

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Antimony nickel titanium rutile

EC Number:

701-459-6

Cas Number:

8007-18-9

Molecular formula:

Ni(x/3)Sb(2x/3)Ti(1-x)O2 0,1≤x≤0,3

IUPAC Name:

Antimony nickel titanium rutile

Test material form:

solid

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method:
C. I. Pigment Yellow 53 was almost insoluble in water and showed a low toxicity in a preliminary test. Consequently, the experiments were carried out with 1.00 mg/L at which precipitates and floating substances were not observed. However, it was likely that the test substance was not dissolved, because the presence of the test substance in the filtrate (the test solution was filtered with 0.45 µm membrane filter) was below the detection limit of ICP-MS operating condition.

Test organisms

Details on test organisms:

TEST ORGANISM
- Common name: Japanese medaka
- Length at study initiation: 1.64 cm (1.51 - 1.80 cm)
- Weight at study initiation: 0.064 g (0.044 - 0.089 g)

ACCLIMATION
- Acclimation period: Acclimated for several days before testing.
- Feeding frequency: Not fed for 24 hours before the test started.
- Health during acclimation: any groups showing > 5 % mortality were not used for testing.

Study design

Test type:

semi-static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Test conditions

Hardness:

Total hardness (as CaCO3): = 63 mg/L;
Alkalinity (as CaCO3): = 43 mg/L

Test temperature:

23.5 - 23.8 ºC

pH:

7.2 - 8.5

Dissolved oxygen:

7.3 - 8.5 mg/L

Nominal and measured concentrations:

control, 1 mg/L (nominal);
1000 mg/L stock solution was prepared for 1.0 mg/L tests, in the following way: 100 mg test substance was added to 100 mL dilution water. The test substance was not dissolved but dispersed in the stock solution.

Details on test conditions:

TEST SYSTEM
- Test vessel: Glass Beaker (5 L)
- Fill volume: 5 L
- Renewal rate of test solution: Every 24 hours
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Dechlorinated tap water
- Conductivity: Electric conductivity: = 150 µS/cm


OTHER TEST CONDITIONS
- Photoperiod: 16:8 hours, light-darkness cycle (<1000 lux)

Results and discussion

Effect concentrations

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 1 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Remarks:

dispersed

Basis for effect:

mortality (fish)

Reported statistics and error estimates:

- Statistical Method: Binomial
a) Data Analysis: The LC50 value and its 95% confidence limits were not determined, a limit test (OECD TG 203) was conducted.
b) Method of Calculating Mean Measured Concentrations (i.e. arithmetic mean, geometric mean, etc.): Geometric Mean

Any other information on results incl. tables

Sublethal observations / clinical signs:

- Measured Concentrations:

The tested concentrations were measured at 0 hour and 24 hours later (before exchange of test solution). The tested concentration at 24 hours was determined to be 71% of nominal concentration; however it was likely that the measured concentration did not reflect dissolved C. I. Pigment Yellow 53 content in the tested solution due to the low water solubility.

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Nominal                      Measured                Mean* measured

concentration             concentration          concentration

mg/L                            mg/L                          mg/L        

 ------------------------------------------------------------------------------------------------           

                    0 Hour (new)       24 Hours (old)    

------------------------------------------------------------------------------------------------

Control               <0.07                 <0.07                    -

1.0                         0.98                   0.71               0.83

------------------------------------------------------------------------------------------------

*: Geometric Mean

 

- Cumulative Mortality:

No death occurred to the tested fish during the test period, except one fish at 96 hr period for 1.00 mg/L (nominal concentration).

------------------------------------------------------------------------------------------------

Nominal           Mean* Meas.              

Concentr.         concentr.                Cumulative Mortality (% Mortality)

mg/L                mg/L                          24hr     48hr    72hr     96hr        

-------------------------------------------------------------------------------------------------

Control            -                                0 (0)     0 (0)     0 (0)     0 (0)

1.00               0.83                            0 (0)      0 (0)    0 (0)    1 (10)

-------------------------------------------------------------------------------------------------

*:Geometric Mean

 

- Other Effect:

No toxicological symptom was observed during the test period.

-------------------------------------------------------------------------------------------------

Nominal          Mean* Meas.             Symptoms

Concentr.       Concentration           (Symptom-number of fish)

mg/L              mg/L                           24hr        48hr        72hr       96hr

-------------------------------------------------------------------------------------------------

Control               -                             Normal   Normal  Normal  Normal

1.00                0.83                           Normal   Normal  Normal  Normal

-------------------------------------------------------------------------------------------------

*: Geometric Mean  

 

- Calculation of toxic values:

The calculation of the toxic values is based on the nominal concentrations.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Antimony nickel titanium rutile is not acutely toxic to fish in a test according to OECD 203 using Japanes medaka (Oryzias latipes) with suspensions prepared with nominal concentrations far exceeding its water solubility and the respective LC50 is estimated with > 1 mg/L (nominal, dispersed).