Antimony nickel titanium rutile

Toxicity to microorganisms

Administrative data

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Remarks:

Rating in accordance with OECD SIDS (2002)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1988

Report date:

1988

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: DIN 38412 Part 27 (draft)

Principles of method if other than guideline:

Bacterial oxygen consumption test according to Robra, K.H., gwf. Wasser-Abwasser 117, 80-86 (1976)

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Antimony nickel titanium rutile

EC Number:

701-459-6

Cas Number:

8007-18-9

Molecular formula:

Ni(x/3)Sb(2x/3)Ti(1-x)O2 0,1≤x≤0,3

IUPAC Name:

Antimony nickel titanium rutile

Test material form:

solid

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

The test solution was stirred for 17 h at 19.85 °C and than used as dispersion (unsoluble in water) in the oxygen consumption test

Test organisms

Test organisms (species):

Pseudomonas putida

Details on inoculum:

- Laboratory culture: yes
Species: Pseudomonas putida
Strain DSM 50026

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

30 min

Test conditions

Test temperature:

24 ± 1 °C

pH:

7.2 - 7.4

Nominal and measured concentrations:

tested concentrations: control, 312.5, 625, 1250, 2500, 5000, 10000 mg/L (nominal)

Details on test conditions:

TEST SYSTEM
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water:
- test volume 100 mL (5 mL glucose [198 g/L] and 95 mL test substance including bacterial suspension)

Results and discussion

Effect concentrations

Effect concentrations 1

Key result

Duration:

30 min

Dose descriptor:

EC10

Effect conc.:

5 680 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Remarks:

dispersed

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Effect concentrations 2

Key result

Duration:

30 min

Dose descriptor:

EC50

Effect conc.:

> 10 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Remarks:

dispersed

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Effect concentrations 3

Duration:

30 min

Dose descriptor:

other: EC90

Effect conc.:

> 10 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Remarks:

dispersed

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Any other information on results incl. tables

The results show the nominal concentrations of the test substance that inhibited oxygen consumption of the microorganisms.

Concentration in mg/L	Mean Oxygen Consumption in % of control
10000	69.2
5000	94.7
2500	100.8
1250	99.2
625	100.8
312	99.2

Applicant's summary and conclusion

Conclusions:

Antimony nickel titanium rutile is not toxic to the microorganisms in a test according to the German standard method DIN 38412 Part 27 with test suspensions prepared at nominal concentrations far exceeding its water solubility. The corresponding EC50 and EC10 for inhibition of oxygen consumption are estimated with > 10000 mg/L and 5680 mg/L (nominal), respectively.