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Toxicity to microorganisms

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Description of key information

Toxicity to microorganisms: 30-min EC50 1000-1800 mg/L (activated sludge)
The inhibition of the degradation activity of activated sludge is not anticipated when introduced in appropriate concentrations.

Key value for chemical safety assessment

EC50 for microorganisms:
1 000 mg/L

Additional information

Activated sludge inhibition data (ISO 8192) are available for ethyl methacrylate and indicate a 30-min EC50 of 1000 -1800 mg/L. Further MAA and the lower alkyl methacrylate category members up to the BMA isomers show a low toxicity to micro-organisms while for 2-EHMA no toxicity has been observed up to and above the limit of solubility. See the following table:

Summary of effects on micro-organisms







Pseudomonas putida

16h EC10= 100 mg/l

16 h growth inhibition test, sensitive species

Hanstveit, 1992


Pseudomonas putida

16h EC3= 100 mg/l

16 h growth inhibition test, sensitive species

Bringmann and Kühn, 1976, 1980ª


Activated sludge

14 d NOEC = 100 mg/l

Ready biodegradability test, OECD 310 C MITI (I)

CITI, 1992


Activated sludge

30 min EC50=
1000- 1800 mg/l

ISO8192 (OECD 209) respiration inhibition test

Hund and Fabig, 1989


Pseudomonas putida

18h EC10
253.6 mg/l

18 h growth inhibition test, sensitive species

Fabig and Hund, 1988


Pseudomonas fluorescens

16h EC10> 281 mg/l*

16 h growth inhibition test

Rump(Fresenius), 1988


Activated sludge

21 d NOEC = 100 mg/l

OECD 301 C

Modified MITI (I) Test

CERI, 1998b

* No growth inhibition with saturated solution