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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Genetic toxicity: in vitro

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Administrative data

Endpoint:
in vitro gene mutation study in mammalian cells
Remarks:
Type of genotoxicity: gene mutation
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Guideline study

Data source

Reference
Reference Type:
other company data
Title:
Unnamed
Year:
2004
Report date:
2004

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 476 (In Vitro Mammalian Cell Gene Mutation Test)
GLP compliance:
yes
Type of assay:
other: thymidine kinase locus

Test material

Constituent 1
Chemical structure
Reference substance name:
Resorcinol
EC Number:
203-585-2
EC Name:
Resorcinol
Cas Number:
108-46-3
Molecular formula:
C6H6O2
IUPAC Name:
resorcinol
Details on test material:
Resorcinol (AO11), >95% purity

Method

Species / strain
Species / strain / cell type:
mouse lymphoma L5178Y cells
Metabolic activation:
with and without
Test concentrations with justification for top dose:
5, 6, 7, 8, 9, and 10 mM (without S9, Experiments 1 and 2)
0.313, 0.625, 1.25, 2.5, 5 and 10 mM (with S9 Experiment 1)
5, 6, 7, 8, 9 and 10 mM (with S9 Experiment 2)
Vehicle / solvent:
The test item was dissolved in dimethylsulfoxide (DMSO).
Controls
Negative solvent / vehicle controls:
yes
Remarks:
Dimethylsulfoxide (DMSO
Positive controls:
yes
Remarks:
Methylmethane sulfonate (MMS); cyclophosphamide (CPA)
Details on test system and experimental conditions:
Type: other: thymidine kinase locus

Results and discussion

Test resultsopen allclose all
Key result
Species / strain:
mouse lymphoma L5178Y cells
Metabolic activation:
without
Genotoxicity:
positive
Cytotoxicity / choice of top concentrations:
not specified
Vehicle controls validity:
not specified
Positive controls validity:
not specified
Key result
Species / strain:
mouse lymphoma L5178Y cells
Metabolic activation:
with
Genotoxicity:
negative
Cytotoxicity / choice of top concentrations:
not specified
Vehicle controls validity:
not specified
Positive controls validity:
not specified

Any other information on results incl. tables

Preliminary Toxicity Study: Without S9:  Following the 3-hour treatment without S9 mix, a moderate to marked toxicity was noted at dose-levels = 1 mM (50-75% decrease in adjusted relative suspension growth (Adj. RSG) and up to 50-69% decrease in adjusted relative total growth (Adj. RTG)).

Main study: 
Without S9:  In both experiments, a moderate to marked toxicity was noted at all dose-levels as shown by 54-76% decrease in Adj. RSG and 46-83% decrease in Adj. RTG.
Noteworthy increases in the mutation frequency (up to 4.8-fold the vehicle control value) were observed following the 3-hour treatment in both experiments.

With S9:

A slight to marked toxicity was observed at dose-levels = 5 mM, as shown by 30-78% decrease in Adj. RSG and 28-65% decrease in Adj. RTG

In the First experiment:  a slight increase in the mutation frequency was noted at the dose-level of 10 mM. Since this very slight increase which did not reach the 2-fold level was neither dose-related nor reproducible in the second experiment, it was not considered as biologically relevant.

Applicant's summary and conclusion

Conclusions:
Interpretation of results: positive

Under these experimental conditions, Resorcinol (A011) induced mutagenic activity in the mouse lymphoma assay, without metabolic activation (S9 mix)