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EC number: 203-585-2 | CAS number: 108-46-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to terrestrial plants
Administrative data
- Endpoint:
- toxicity to terrestrial plants: long-term
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 4 (not assignable)
Data source
Reference
- Reference Type:
- publication
- Title:
- Identification and phytotoxic activity of compounds produced during decomposition of corn and rye residues in soil.
- Author:
- Chou C.H.and Patrick ZA
- Year:
- 1 976
- Bibliographic source:
- Journal of Chemical Ecology 2(3):369-387. With kind permission from Springer Science and Business Media
Materials and methods
Test guideline
- Guideline:
- other:
- Principles of method if other than guideline:
- The main purpose of the study was to identify compounds formed during the decomposition of corn and rye in soil. Because of the important role of phytotoxins, the isolation and identification was confined mainly to substances with phytotoxic properties.
- GLP compliance:
- not specified
Test material
- Reference substance name:
- Resorcinol
- EC Number:
- 203-585-2
- EC Name:
- Resorcinol
- Cas Number:
- 108-46-3
- Molecular formula:
- C6H6O2
- IUPAC Name:
- resorcinol
Constituent 1
Test organisms
- Species:
- Lactuca sativa
- Details on test organisms:
- Lettuce seeds, Lactuca sativa (var Great Lakes)
Study design
- Test type:
- other: Determination of phytotoxicity
- Study type:
- laboratory study
- Substrate type:
- filter paper
- Total exposure duration:
- 30 d
Test conditions
- Test temperature:
- 21 – 22C
- Reference substance (positive control):
- not specified
Results and discussion
Effect concentrations
- Remarks on result:
- other: See section Any other information on results
- Remarks:
- See section Any other information on results
- Details on results:
- In the lettuce seed assay, most of the compounds from the corn deposition products exhibited phytotoxicity (including resorcinol). In resorcinol a significant reduction in growth was observed at 100 ppm.
- Results with reference substance (positive control):
- Control: growth of lettuce was normal.
Control: Series consisted of soil without added crop residue. - Reported statistics and error estimates:
- Statistical Analysis: Student’s t-test.
Any other information on results incl. tables
The phytotoxicity of aqueous extracts obtained from the decomposing corn residues was determined using the volatile bioassay method. The test seeds received the volatiles from the surrounding extracts held by sponges without direct contact with the extract.
Toxicities tended to decrease with the second extraction and were almost lost after the fifth extraction. It appears that the phytotoxicity of the extracts is mainly on the amount of organic substrate decomposing in the soil. The toxicity decreased as the amounts of soil incorporated into the corn residue was increased or as the decomposition period was increased.
Chromatographic bioassay of Phytotoxic extracts:
By using the chromatographic bioassay, the phytotoxicity was determined on 30µl (about 1.5% concentration of extracts) of the ether fraction or each extract. Most of the toxic spots were located in the zone between 0.37 and 0.97. Two distinguishable toxic spots were located on the chromatogram at Rf 0.63 – 0.73 and at Rf 0.73 – 0.84. At these two spots not only was lettuce seed germination inhibited but radical growth was also suppressed. Toxicity was maximum after 25 – 30 days’ decomposition, which is consistent with the results obtained in the volatile assay. As more soil was incorporated with the corn residue, the toxicity was decreased. The chromatograph bioassay of three subsequent extracts was also performed. At 25 days decomposition, the toxic spots remained in the Rf 0.63 – 0.84 zone. In the third extracts, however, the toxicity was almost lost in both the 1:1 and 1:2 corn to soil ratios.
The toxic spots were further analyzed. Eighteen compounds were identified in which it was determined that resorcinol was not considered to be volatile under the test conditions. In the chromatographic assay, resorcinol was identified as one of the four toxic zones. An attempt was made to determine the concentration levels of the various phytotoxins in the decomposing corn residues. The results were inconclusive, but it appeared that the concentration ranged between 5 and 100 µg/g soil.
Results of the bioassay conducted in the synthetic chemical, resorcinol were compared to those when testing the corn residue (of which resorcinol was detected and labeled as phytoxin 9).
The following results are available:
Gas chromatography |
Paper chromatography |
|||||||
Retention |
Rf values (x100) |
Colour Reaction |
||||||
distance |
(mm) |
2% AA |
BuAW |
BzAW |
sUV |
DPNA |
DQC |
|
Resorcinol |
188 |
70 |
86 |
38 |
ab |
yel |
gr pu |
|
Phytotoxin 9 |
188 |
67 |
85 |
40 |
ab |
yel |
gr pu |
*absorption; yel = yellow; gr = gray; pu = purple
Relative Concentration of Phytotoxin in 3 treated Corn-Soil mixtures
Decomposition Period |
|||||||||
5 days |
15 days |
30 days |
|||||||
1:0 |
1:1 |
1:2 |
1:0 |
1:1 |
1:2 |
1:0 |
1:1 |
1:2 |
|
Resorcinol |
+ |
+ |
+ |
+ the concentration was determined using gas chromatograph and determined to be 5 µg/g soil
Effects of known compounds in aqueous solution on radical growth of Lactuca sativa after 72 hours at 21 – 22C
Radicle growth (% of control) |
||||
Concentration (ppm) |
||||
100 |
200 |
300 |
400 |
|
resorcinol |
63 |
49 |
41 |
25 |
Results were significantly different from the control below the 5% level of confidence.
Applicant's summary and conclusion
- Conclusions:
- In the case of resorcinol 100% growth inhibition was observed. The seeds exposed to the toxic atmosphere from the extracts were damaged, and some turned black or dark brown. Some of the seeds were also swollen. In addition, the radical was abnormal and lacked root hairs, and there was necrosis of the root tips.
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