2-nitrotoluene

Long-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

long-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

other information

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

documentation insufficient for assessment

Remarks:

Comparable to guideline study with acceptable restrictions, Basic data given

Principles of method if other than guideline:

- in analogy with the OECD proposal (1979) long-term toxicity test were performed on crustaceans

GLP compliance:

not specified

Specific details on test material used for the study:

- Name of test material (as cited in study report): 2-Nitrotoluene
- Analytical purity: > 99.5%
- Supplier: Fluka

Test organisms (species):

Daphnia magna

Duration:

21 d

Dose descriptor:

LC50

Effect conc.:

7.5 mg/L

Duration:

21 d

Dose descriptor:

EC50

Effect conc.:

2.6 mg/L

Basis for effect:

other: mortality and behavior

Duration:

21 d

Dose descriptor:

EC50

Effect conc.:

2.5 mg/L

Basis for effect:

reproduction

Duration:

21 d

Dose descriptor:

NOEC

Effect conc.:

0.5 mg/L

Basis for effect:

reproduction

EU Risk Assessment (2008):

 

Canton et al. (1985) determined the following values for Daphnia magna after a semi-chronic exposure period: 2.5 mg/l as the concentration that reduced the reproduction in a 50% and, 0.5 mg/l as the no observed inhibition of reproduction.

Executive summary:

Canton et al. (1985) determined the following values for Daphnia magna after a semi-chronic exposure period (21 days) with 2-nitrotoluene in analogy with the OECD proposal (1979) long-term toxicity test were performed on crustaceans: LC50: 7.5 mg/L, EC50 (mortality and behavior): 2.6 mg/L, EC50 (reproduction): 2.5 mg/L and NOEC (reproduction): 0.5 mg/L.

Reference 2

Endpoint:

long-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

other information

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

documentation insufficient for assessment

Remarks:

National Guideline study with acceptable restrictions

Qualifier:

according to guideline

Guideline:

other: protocol of the Dutch Standards Organisation NEN 6502 (1980)

GLP compliance:

not specified

Specific details on test material used for the study:

Name of test material (as cited in study report): 2-Nitrotoluene
- Analytical purity: > 99.5%
- Supplier: Fluka

Test organisms (species):

Daphnia magna

Details on test organisms:

- all daphnids used were <24 h old at the start of the experiment
- during the test daphnids were fed with Chlorella pyrenoidosa 3x10E8 cells l-1 day-1

Test type:

semi-static

Water media type:

freshwater

Total exposure duration:

21 d

Hardness:

200 mg/l CaCO3

Test temperature:

20 ± 0.5°C

pH:

8.4 ± 0.1°C

Dissolved oxygen:

synthetic test medium saturated with air prior to use

Details on test conditions:

- illumination: 12 h/d

Duration:

21 d

Dose descriptor:

IC50

Effect conc.:

7.36 mg/L

Basis for effect:

immobilisation

Duration:

21 d

Dose descriptor:

LOEC

Effect conc.:

9.93 mg/L

Basis for effect:

reproduction

Duration:

21 d

Dose descriptor:

LOEC

Effect conc.:

9.93 mg/L

Basis for effect:

growth

EU RAR (2008):

 

Deneer et al. (1989) carried out a study on D. magna, in semi-static test conditions, following the protocol of the Dutch Standards Organization NEN 6502 (1980). Test organisms were fed with 3·10⁸cells/l·day of Chlorella pyrenoidosa. All daphnids used were < 24 h old at the start of the experiments. A log(21d-LOEC) of 1.86 µmol/l (21d-LOEC of 9.9 mg/l) was determined for 21-d test as the lowest concentration tested that significantly decreased the length and the intrinsic growth rate of Daphnia magna population.

Executive summary:

Deneer et al. (1989) carried out a study on Daphnia magna, in semi-static test conditions, following the protocol of the Dutch Standards Organization NEN 6502 (1980). Test organisms were fed with 3·108cells/l·day of Chlorella pyrenoidosa. All daphnids used were < 24 h old at the start of the experiments. A log(21d-LOEC) of 1.86 µmol/l (21d-LOEC of 9.9 mg/l) was determined for 21-d test as the lowest concentration tested that significantly decreased the length and the intrinsic growth rate of Daphnia magna population.

Description of key information

For transported isolated intermediates according to REACh, Article 18, this endpoint is not a data requirement. However, data is available for this endpoint and is thus reported under the guidance of "all available data".

Canton et al. (1985) determined the following values for Daphnia magna after a semi-chronic exposure period (21 days) with 2-nitrotoluene in analogy with the OECD proposal (1979) long-term toxicity test were performed on crustaceans: LC50: 7.5 mg/L, EC50 (mortality and behavior): 2.6 mg/L, EC50 (reproduction): 2.5 mg/L and NOEC (reproduction): 0.5 mg/L.

Deneer et al. (1989) carried out a study on Daphnia magna, in semi-static test conditions, following the protocol of the Dutch Standards Organization NEN 6502 (1980). Test organisms were fed with 3·108cells/l·day of Chlorella pyrenoidosa. All daphnids used were < 24 h old at the start of the experiments. A log(21d-LOEC) of 1.86 µmol/l (21d-LOEC of 9.9 mg/l) was determined for 21-d test as the lowest concentration tested that significantly decreased the length and the intrinsic growth rate of Daphnia magna population.