1,2-dichloropropane

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Study period:

November 29 - December 02, 2010

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2010

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

Test guideline 2

Qualifier:

according to guideline

Guideline:

EPA OPPTS 850.5400 (Algal Toxicity, Tiers I and II) (January 2012)

Test guideline 3

Qualifier:

according to guideline

Guideline:

EU Method C.3 (Algal Inhibition test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

1,2-dichloropropane

EC Number:

201-152-2

EC Name:

1,2-dichloropropane

Cas Number:

78-87-5

Molecular formula:

C3H6Cl2

IUPAC Name:

1,2-dichloropropane

Details on test material:

Name: 1,2-dichloropropane

Sampling and analysis

Analytical monitoring:

yes

Test organisms

Test organisms (species):

Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

72 h

Test conditions

Test temperature:

The temperature was in the range of 22.7 – 22.9 °C measured in the flask and between 21.8 and 23.1 °C measured within the climate chamber.

pH:

The range of the pH was 7.24 – 7.90 at the start and 8.12 – 8.72 at the end of the study

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

> 7.95 mg/L

Conc. based on:

test mat.

Basis for effect:

growth rate

Effect concentrations 2

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

7.95 mg/L

Conc. based on:

test mat.

Basis for effect:

growth rate

Effect concentrations 3

Duration:

72 h

Dose descriptor:

LOEC

Effect conc.:

> 7.95 mg/L

Conc. based on:

test mat.

Basis for effect:

growth rate

Any other information on results incl. tables

The cell density in the control cultures increased by a factor of 68.17 withinthree days.

The mean coefficient of variation for section-by-section specific growth rates
(days 0-1; 1-2; 2-3) in the control cultures was 11.65 %.

The coefficient of variation of average specific growth rates during the whole test period (day 0-3) in the control cultures was 1.30 %.

All validity criteria were met, therefore the study can be considered as valid.

Applicant's summary and conclusion

Conclusions:

Under the conditions of this algal growth inhibition test, no toxic effect was observed up to the maximum achieved concentration level of 7.95 mg/L.
Correspondingly the No Observed Effect Concentration was determined as 7.95 mg/L, the 72h EC50 values were higher than 7.95 mg/L.