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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Genetic toxicity: in vitro

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Administrative data

Endpoint:
in vitro gene mutation study in bacteria
Remarks:
mitotic recombination in Saccharomyces cerevisiae
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)

Data source

Reference
Reference Type:
publication
Title:
Unnamed
Year:
1979

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
The mutagenicity of lead chromate was examined in a battery of microbial tests including: the E. coli PolA+/PolA- survival test, the Salmonella/microsome His+ reversion assay, the E. coli Trp+ reversion test, the E. coli Gal+ forward mutation test, and the S. cerevisiae mitotic recombination assay.
GLP compliance:
not specified
Type of assay:
other: bacterial gene mutation assay; mitotic recombination in Saccharomyces cerevisiae

Test material

Constituent 1
Reference substance name:
Lead chromate
EC Number:
231-846-0
EC Name:
Lead chromate
IUPAC Name:
Lead chromate

Results and discussion

Test results
Species / strain:
other: S. typhimurium; E. coli; Saccharomyces cerevisiae
Metabolic activation:
with and without
Genotoxicity:
positive
Cytotoxicity / choice of top concentrations:
cytotoxicity
Vehicle controls validity:
valid
Untreated negative controls validity:
valid
Positive controls validity:
valid
Remarks on result:
other: Test system: all strains/cell types tested

Any other information on results incl. tables

Lead chromate was positive for mutations in the S. typhimurium His+ reversion assay (at concentrations from 0.2 to 0.4 mg/plate in the presence and absence of S9) and the E. coli Trp+ reversion fluctuation test (at concentrations from 5 uM to 20 uM). Lead chromate was also positive for recombinations in the S. cerevisiae D5 assay at concentrations from 62.5 to 250 ug/ml. Lead chromate had negative responses in the E.coli PolA-/PolA+ assay (at concentrations of 0.2 and 0.3 mg/plate), the E.coli Gal+ forward mutation test (at concentrations from 2.5 to 100 mg/ml), and the E.coli Trp+ reversion plate test (at concentrations of 4 to 60 uM). The authors suggested that the chromate ion is responsible for the observed mutagenicity in Salmonella.

Applicant's summary and conclusion

Conclusions:
Interpretation of results: positive
Executive summary:

The mutagenicity of lead chromate was examined in a battery of microbial tests including: the E. coli PolA+/PolA- survival test, the Salmonella/microsome His+ reversion assay, the E. coli Trp+ reversion test, the E. coli Gal+ forward mutation test, and the S. cerevisiae mitotic recombination assay. Lead chromate was positive for mutations in the S. typhimurium His+ reversion assay (at concentrations from 0.2 to 0.4 mg/plate in the presence and absence of S9) and the E. coli Trp+ reversion fluctuation test (at concentrations from 5 uM to 20 uM). Lead chromate was also positive for recombinations in the S. cerevisiae D5 assay at concentrations from 62.5 to 250 ug/ml. Lead chromate had negative responses in the E.coli PolA-/PolA+ assay (at concentrations of 0.2 and 0.3 mg/plate), the E.coli Gal+ forward mutation test (at concentrations from 2.5 to 100 mg/ml), and the E.coli Trp+ reversion plate test (at concentrations of 4 to 60 uM). The authors suggested that the chromate ion is responsible for the observed mutagenicity in Salmonella.