Registration Dossier

Administrative data

Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Study period:
October 2014 - November 2014
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2015
Report Date:
2015

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
Deviations:
no
GLP compliance:
yes (incl. certificate)

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
Batch identification: MKBR7485V
Barium: 88.7 g/ 100 g

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
activated sludge of a predominantly domestic sewage
Details on inoculum:
Origin: aeration tankof the wastewater treatment plant of Mannheim, Germany

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
3 h

Test conditions

Test temperature:
19.9 - 20.5 °C
pH:
8.7 - 12.3 Measured before correction)
7.2 - 7.5 (corrected before addition of inocolum)
7.9 - 8.1 (after incubation time
Dissolved oxygen:
> 7 mg/L (immediately before measurements)
Nominal and measured concentrations:
Nominal concentrations: 1000 mg/L, 500 mg/l, 250 mg/L, 125 mg/L, 62.5 mg/L
Details on test conditions:
Test replicates: 6 replicates for the control,
3 replicates for each test substance concentration,
2 replicates for each reference substance concentration.
Reference substance (positive control):
yes
Remarks:
3,5 dichlorophenol

Results and discussion

Effect concentrationsopen allclose all
Duration:
3 h
Dose descriptor:
EC10
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Basis for effect:
other: respiration inhibition
Duration:
3 h
Dose descriptor:
other: EC20
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Basis for effect:
other: respiration inhibition
Duration:
3 h
Dose descriptor:
EC50
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Basis for effect:
other: repiration inhibition
Duration:
3 h
Dose descriptor:
other: EC80
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Basis for effect:
other: respiration inhibition
Results with reference substance (positive control):
The EC50 of 3,5-dichlorophenol was 8.5 mg/L

Applicant's summary and conclusion

Validity criteria fulfilled:
yes