Registration Dossier
Registration Dossier
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EC number: 201-058-1 | CAS number: 77-78-1
Skin sensitisation
Administrative data
- Endpoint:
- skin sensitisation: in vivo (LLNA)
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Meets generally accepted scientific standards
Data source
Reference
- Reference Type:
- publication
- Title:
- Structure activity relationships in a skin sensitization using the murine local lymph node assay
- Author:
- Ashby J, Basketter DA, Paton D AND Kimber I
- Year:
- 1 995
- Bibliographic source:
- Toxicology 103: 177-194
Materials and methods
- Principles of method if other than guideline:
- Proliferation of local lymph node was determined by means of increased 3H-thymidine incorporation. A threefold increase compared with vehicle-treated controls was considered to be determinant of sensitising capacity.
- GLP compliance:
- not specified
- Type of study:
- mouse local lymph node assay (LLNA)
Test material
- Reference substance name:
- Automatically generated during migration to IUCLID 6, no data available
- IUPAC Name:
- Automatically generated during migration to IUCLID 6, no data available
- Details on test material:
- - Name of test material (as cited in study report): Dimethyl sulfate
No further specification
Constituent 1
In vivo test system
Test animals
- Species:
- mouse
- Strain:
- not specified
- Sex:
- not specified
- Details on test animals and environmental conditions:
- No further data
Study design: in vivo (LLNA)
- Vehicle:
- acetone/olive oil (4:1 v/v)
- Concentration:
- 0.25, 0.5, and 1.0% in acetone/olive oil 80/20 v/v
- No. of animals per dose:
- number of animals not indicated
- Details on study design:
- MAIN STUDY
ANIMAL ASSIGNMENT AND TREATMENT
- Name of test method: LLNA
- Criteria used to consider a positive response: a threefold increase compared with vehicle-treated controls was considered to be determinant of sensitising capacity
TREATMENT PREPARATION AND ADMINISTRATION:
Topical dosing of test substance - Positive control substance(s):
- other: 106 chemicals are listed in the evaluation, some of which are known sensitizing agents
- Statistics:
- topical dosing of test substance (see below)
Results and discussion
In vivo (LLNA)
Resultsopen allclose all
- Parameter:
- SI
- Remarks on result:
- other: Ratio of 3H-thymidine incorporation in local lymph nodes of treated vs control animals was reported to be 3.8 (concentration of test substance 0.25%), 6.0 (0.5%), 5.7 (1.0%).
- Parameter:
- other: disintegrations per minute (DPM)
- Remarks on result:
- other: not specified
Applicant's summary and conclusion
- Interpretation of results:
- sensitising
- Remarks:
- Migrated information
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