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Toxicological information

Skin irritation / corrosion

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Administrative data

Endpoint:
skin irritation: in vivo
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Meets generally acceptad scientific standards, well documented and acceptable for assessment

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1968
Report Date:
1968

Materials and methods

Test guideline
Qualifier:
equivalent or similar to
Guideline:
OECD Guideline 404 (Acute Dermal Irritation / Corrosion)
Deviations:
yes
Remarks:
- Animals were treated for 1, 5, and 15 min or 20 hours using occlusive conditions; - Skin tissue from the ear was also tested; - The test substance was washed out with water (which sometimes contained a mild detergent) after the exposure period.
Principles of method if other than guideline:
BASF-Test
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): dimethyl sulfate
- Physical state: liquid

Test animals

Species:
rabbit
Strain:
Vienna White
Details on test animals and environmental conditions:
TEST ANIMALS
- Age at study initiation: young adult animals
- Weight at study initiation: 2.35 kg (female; 2.46 kg at the end of the study) and 2.5 kg (male; 2.36 kg at the end of the study)

Test system

Type of coverage:
occlusive
Preparation of test site:
other: clipped and intact skin
Vehicle:
unchanged (no vehicle)
Controls:
other: untreated skin of the same animal
Amount / concentration applied:
TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 0.5-1 ml
Duration of treatment / exposure:
1 min, 5 min, 15 min and 20 hours
Observation period:
8 days
Number of animals:
2
Details on study design:
TEST SITE
- Area of exposure: undiluted test substance was applied to a 2.5 x 2.5 cm patch
- Type of wrap if used: occlusive

REMOVAL OF TEST SUBSTANCE
- Washing (if done): yes
- Time after start of exposure: up to 20 hours after treatment

SCORING SYSTEM:
Dermal reactions were classified using a BASF-scoring system, which is convertible into the current OECD Draize scheme.

Results and discussion

In vivo

Results
Irritation parameter:
other: the test substance is corrosive
Basis:
mean
Reversibility:
not reversible
Remarks on result:
other: necrosis was still observable at the end of the exposure period (8-day)
Irritant / corrosive response data:
Short time exposure caused no effects (1 min exposure) or light erythema (score: 2; 5 and 15 min exposure): Cutaneous reactions were reversible after 1 and 5 min exposure, but not after 15 min exposure at the end of the observation period.
Long time exposure (20 hours): Severe cutaneous reaction (erythema score 4 and edema score 3) and severe necrosis of skin tissue was reported for both animals 24 hours after application of the test substance, which persisted until the end of the observation period (8 days after application of the test substance).

Any other information on results incl. tables

Conclusion:

The test substance is considered to be corrosive based on the irreversible necrosis

Applicant's summary and conclusion

Interpretation of results:
corrosive
Remarks:
Migrated information