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Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
data from handbook or collection of data

Data source

Reference
Reference Type:
publication
Title:
BUA-Stoffbericht 265 - 2-Chlorbezonitril, 4-Chlorbenzonitril
Author:
n.a.
Year:
2006
Bibliographic source:
Beratergremium für Altstoffe (BUA) der Gesellschaft Deutscher Chemiker, S.Hirzel Wissenschaftliche Verlagsgesellschaft

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 201 (Alga, Growth Inhibition Test)
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
2-chlorobenzonitrile
EC Number:
212-836-5
EC Name:
2-chlorobenzonitrile
Cas Number:
873-32-5
Molecular formula:
C7H4ClN
IUPAC Name:
2-chlorobenzonitrile
impurity 1
Reference substance name:
unknown
IUPAC Name:
unknown
Test material form:
solid

Sampling and analysis

Analytical monitoring:
not specified

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Chlorella vulgaris

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
6 h

Results and discussion

Effect concentrationsopen allclose all
Key result
Duration:
6 h
Dose descriptor:
EC50
Effect conc.:
54 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
biomass
Remarks on result:
other: photometric determination of biomass at wavelength of 680 nm
Key result
Duration:
6 h
Dose descriptor:
EC50
Effect conc.:
43 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
biomass
Remarks on result:
other: photometric determination of biomass at wavelength of 750 nm

Applicant's summary and conclusion

Validity criteria fulfilled:
not specified
Conclusions:
The 6-h EC50 (basis for effect: biomass) of 2-chlorobenzonitrile for Chlorella vulgaris was determined to be 54 mg/L (photometric determination of wavelength at 680 nm).
Executive summary:

The effect of 2-chlorobenzonitrile on reduction of biomass of Chlorella vulgaris was determined photometrically at 680 nm and 750 nm. No data are available on the concentrations tested. Chlorella vulagris was used in aqueous solution of the test substance at approximately 7.5 x 10^8 cells/ml. The cell suspensions were incubated for 6 hours at 36.5 °C at an illumination intensity of 28 W/m².

The 6-h EC50 was determined to be 54 mg/L and 43 mg/L at 680 nm and 750 nm, respectively.

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