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Toxicological information

Eye irritation

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Administrative data

Endpoint:
eye irritation: in vivo
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1990
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1990
Report date:
1990

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 405 (Acute Eye Irritation / Corrosion)
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
2-chlorobenzonitrile
EC Number:
212-836-5
EC Name:
2-chlorobenzonitrile
Cas Number:
873-32-5
Molecular formula:
C7H4ClN
IUPAC Name:
2-chlorobenzonitrile
impurity 1
Reference substance name:
unknown
IUPAC Name:
unknown
Test material form:
solid

Test animals / tissue source

Species:
rabbit
Strain:
New Zealand White

Test system

Vehicle:
unchanged (no vehicle)
Controls:
yes
Duration of treatment / exposure:
Single instillation of 0.1 ml of the test substance (approx. 90 mg of 2-Chlorobenzonitrile) into the lower everted eye lid of one eye of each animal.
Observation period (in vivo):
Examination of the eyes was made after 1 hour and 1, 2, 3, 4 and 7 days after instillation
Number of animals or in vitro replicates:
3 females

Results and discussion

In vivo

Resultsopen allclose all
Irritation parameter:
cornea opacity score
Basis:
animal: 813
Time point:
24/48/72 h
Score:
0
Max. score:
0
Reversibility:
not specified
Irritation parameter:
iris score
Basis:
animal: 813
Time point:
24/48/72 h
Score:
0
Max. score:
0
Reversibility:
not specified
Irritation parameter:
conjunctivae score
Basis:
animal: 813
Time point:
24/48/72 h
Score:
>= 1 - < 2
Max. score:
2
Reversibility:
fully reversible within: 2 days
Irritation parameter:
chemosis score
Basis:
animal: 813
Time point:
24/48/72 h
Score:
>= 0 - <= 1
Max. score:
1
Reversibility:
fully reversible within: 2 days
Irritation parameter:
cornea opacity score
Basis:
animal: 840
Time point:
24/48/72 h
Score:
>= 1 - <= 2
Max. score:
2
Reversibility:
fully reversible within: 4 days
Irritation parameter:
iris score
Basis:
animal: 840
Time point:
24/48/72 h
Score:
0
Max. score:
0
Reversibility:
not specified
Irritation parameter:
conjunctivae score
Basis:
animal: 840
Time point:
24/48/72 h
Score:
>= 1 - <= 2
Max. score:
2
Reversibility:
fully reversible within: 2 days
Irritation parameter:
chemosis score
Basis:
animal: 840
Time point:
24/48/72 h
Score:
>= 0 - <= 1
Max. score:
1
Reversibility:
fully reversible within: 1 day
Irritation parameter:
cornea opacity score
Basis:
animal: 841
Time point:
24/48/72 h
Score:
1
Max. score:
1
Reversibility:
fully reversible within: 7 days
Irritation parameter:
iris score
Basis:
animal: 841
Time point:
24/48/72 h
Score:
0
Max. score:
0
Reversibility:
not specified
Irritation parameter:
conjunctivae score
Basis:
animal: 841
Time point:
24/48/72 h
Score:
>= 1 - <= 2
Max. score:
2
Reversibility:
fully reversible within: 2 days
Irritation parameter:
chemosis score
Basis:
animal: 841
Time point:
24/48/72 h
Score:
>= 0 - <= 1
Max. score:
1
Reversibility:
fully reversible within: 1 day

Any other information on results incl. tables

Ocular reactions elicited by 2-Chlorobenzonitrile

Rabbit Number and sex

Body weight

(Day 1)

Region of Eye

One hour

Day after instillation

1

2

3

4

7

813

female

2665 g

Cornea

Density

0

0

0

0

0

0

Area

0

0

0

0

0

0

Iris

0

0

0

0

0

0

Conjunctivae

Redness

2

1

0

0

0

0

Chemosis

1

1

0

0

0

0

840

female

2770 g

Cornea

Density

0

2

2

1

0

0

Area

0

1

1

1

0

0

Iris

0

0

0

0

0

0

Conjunctivae

Redness

2

1

0

0

0

0

Chemosis

1

0

0

0

0

0

841

female

3020 g

Cornea

Density

0

1

1

1

1

0

Area

0

1

1

1

1

0

Iris

0

0

0

0

0

0

Conjunctivae

Redness

2

1

0

0

0

0

Chemosis

1

0

0

0

0

0

 

Applicant's summary and conclusion

Interpretation of results:
GHS criteria not met
Conclusions:
2-Chlorobenzonitrile elicited transient, corneal opacification and temporary well-defined conjunctival reactions.
Executive summary:

In a GLP guideline study according to OECD 405 (1987) 0.1 ml (approx. 90 mg) 2-Chlorobenzonitrile was installed into the lower everted eye lid of one eye of each of 3 female New Zealand White rabbits. Examination of the eyes was made after 1 hour and 1, 2, 3, 4 and 7 days after instillation. All animals were observed daily for signs of ill health or toxic signs.The test material elicited transient, corneal opacification and temporary well-defined conjunctival reactions.

Two of three animals show corneal effects >=1 after 24 -72h. Based on the scoring result the classification “H319: Causes serious eye irritation” is warranted.

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